GRADE & PURITYRecombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.Validated?Validated grade — performance confirmed in defined applications before release. Use when you want documented assurance the reagent works as stated.ExactAb™?ExactAb™ — Aladdin's antibody line emphasizing precision and validated quality. Use for reliable, specificity-validated antibody applications.0.5 mg/mL
Functions as a coreceptor for the B-cell antigen receptor complex (BCR) on B-lymphocytes.
Decreases the threshold for activation of downstream signaling pathways and for triggering B-cell responses to antigens.
Activates signaling pathways that lead to th
Host species
Mouse
Specificity
Human CD19
Immunogen
NALM1 + NALM16 cells
Positive Control
Flow Cytometry: Human peripheral blood lymphocytes
Conjugation
Biotin
Grade
ExactAb™, Recombinant, Validated
Light Chain Type
kappa
Purification Method
Protein A purified
Concentration
0.5 mg/mL
Storage and Shipping
Storage
Store at 2-8°C,Protected from light,Do not freeze
Shipped In
Wet ice,Do not freeze
Stability And Storage
The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.
Images
Recombinant CD19 Antibody (Biotin) (Ab223514) - Flow Cytometry Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (right panel) compared to an isotype control (left panel). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (Biotin) (Ab223514) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Left panel - Mouse IgG (Biotin) (Ab180306).
Recombinant CD19 Antibody (Biotin) (Ab223514) - Flow Cytometry Flow cytometry analysis of CD19 expression by human peripheral blood lymphocytes (red) compared to an isotype control (blue). Human peripheral blood cells were preincubated with Human Fc Receptor Blocking Solution (H1374078). The cells were then stained with Recombinant CD19 Antibody (Biotin) (Ab223514) at 0.1μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Recombinant CD19 Antibody (Biotin) (Ab223514) - Flow Cytometry Flow cytometry analysis of CD19 expression by Raji cells (red) compared to an isotype control (blue). Cells were stained with Recombinant CD19 Antibody (Biotin) (Ab223514) at 0.3μg/test for 1 hour at 4°C. Then incubated with Recombinant Streptavidin Protein (R-PE) (rp156150) at 0.1μg/test for 30 minutes at 4°C in the dark. Blue - Mouse IgG (Biotin) (Ab180306). Black - Unlabelled control, cells without incubation with primary antibody.
Application
Application
Dilution info
Flow Cytometry
≤1.0μg/ test
For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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