Recombinant Human Copper-Zinc Superoxide Dismutase (rh-Cu/Zn-SOD), CAS No.9054-89-1

CAS: 9054-89-1 Cat. No.: R1523652 EC Number: 232-943-0

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), ≥1000 U/mg enzyme powder; expressed in Pichia pastoris

Accession #

P00441

Expression system

Pichia pastoris

Endotoxin Concentration

<0.2 EU/μg

Bioactivity

≥1000 U/mg enzyme powder

Overview

Superoxide Dismutase (SOD), also known as orgotein, is a bio-derived active substance that eliminates harmful substances produced during metabolic processes in organisms. With free-radical-scavenging activity, SOD exerts anti-aging, immunomodulatory, lipid-regulating, blood-glucose-regulating, anti-radiation and skin-beautifying effects.

This product is recombinant human copper-zinc superoxide dismutase (rh-Cu/Zn-SOD). It is produced by fermentation of engineered Komagataella phaffii (formerly Pichia pastoris), followed by separation, purification and lyophilization to obtain lyophilized powder.

Product Advantages

Animal-origin free: Free of animal viruses, pathogenic substances and exogenous factor contamination, featuring high safety.

High purity: Elevated specific activity; residual host protein content complies with the limits specified for biological products.

Complies with the standards of Chinese Pharmacopoeia 2020 Edition and USP 43.

Compliance: Production facilities and environment meet relevant regulatory requirements and GMP guidelines.

Complete quality documentation: Relevant regulatory supporting documents are available upon customer request.

Recommended Application Methods

Cosmetics: Add directly to the aqueous phase of cosmetic formulations at a temperature below 40 °C in the final preparation stage. The recommended dosage is 100 U–1000 U per gram of cosmetic base.Other applications: Dosage can be adjusted as appropriate.

Product Applications

Cosmetics: Absorbs ultraviolet rays to deliver prominent sun protection. Effectively scavenges free radicals to prevent skin aging, reduces melanin accumulation and fades pigmentation spots. It also inhibits sebum secretion and relieves acne.

Health supplements: Eliminates free radicals and harmful substances to boost human immunity.

Food: Possesses anti-inflammatory properties, and can be used as a food preservative as well as a fresh-keeping agent for fruits and vegetables.

Transportation & Storage Stability

Transportation: Shipped with ice packs to maintain constant activity during transit.

Storage: Store at −15 ~ −20 °C; stable for up to 24 months. When formulated into solutions with enzyme concentration above 1 mg/mL using Tris-HCl buffer (pH 6.0–8.0), the product remains stable for at least 48 hours at 4 °C.

Precautions & Disclaimer

This product is hygroscopic. Please let it equilibrate to room temperature before use.

This product is for research and production use only. It shall not be used for the diagnosis or treatment of humans and animals, and is prohibited from direct application to human bodies.

Specifications

Product Name

Recombinant Human Copper-Zinc Superoxide Dismutase (rh-Cu/Zn-SOD), CAS No.9054-89-1

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥95%(SDS-PAGE), ≥1000 U/mg enzyme powder; expressed in Pichia pastoris

Biochemical and Physiological Mechanisms

Destroys radicals which are normally produced within the cells and which are toxic to biological systems.Catalyzes the oxidation of hydrogen sulfide (H2S) to sulfate, playing an important role in detoxifying H2S and limiting the accumulation of reactive s

Bioactivity

≥1000 U/mg enzyme powder

Endotoxin Concentration

<0.2 EU/μg

Accession #

P00441

SDS-PAGE

14.3-17.5 kDa

CAS

9054-89-1

Enzyme Commission Number

EC: 1.15.1.1

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥1000 U/mg enzyme powder; expressed in Pichia pastoris

Reconstitution

Reconstitute in sterile water or physiological saline to a concentration of 100U-1000 U/ml.

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (24 months). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Unit definition

One unit (1 U) of SOD is defined as the enzyme amount required to inhibit 50 % of the autoxidation rate of pyrogallol at 25 °C

References

1. Dong Zhicheng, Xu Yunyun, Wu Can, Chao Jin, Tian Chen, Lin Zhang.  (2023)  Efficient removal of natural organo-chromium(III) through self-circulating decomplex and immobilization with nanoscale zero-valent iron.  Nano Research,      [PMID:] [10.1007/s12274-023-6028-9]

2. Jiayi Luo, Yunqiang Yi, Zhanqiang Fang.  (2023)  Nitrogen-rich magnetic biochar prepared by urea was used as an efficient catalyst to activate persulfate to degrade organic pollutants.  CHEMOSPHERE,      [PMID:37482309] [10.1016/j.chemosphere.2023.139614]

3. Jiayi Luo, Yunqiang Yi, Zhanqiang Fang.  (2023)  Effect of Mn-based magnetic biochar /PS reaction system on oxidation of metronidazole.  CHEMOSPHERE,      [PMID:37119924] [10.1016/j.chemosphere.2023.138747]

4. Gu Yurong, Zhang Yi, Jiang Chengchun, Dong Zijun, Bai Xue.  (2023)  Efficient metformin transformation in sulfite/UV process co-present with oxygen.  Frontiers in Environmental Science,      [PMID:] [10.3389/fenvs.2022.1071963]

5. Xixi Chen, Wanyi Fu, Zhichao Yang, Yulong Yang, Yanjun Li, Hui Huang, Xihui Zhang, Bingcai Pan.  (2023)  Enhanced H2O2 utilization efficiency in Fenton-like system for degradation of emerging contaminants: Oxygen vacancy-mediated activation of O2.  WATER RESEARCH,      [PMID:36603306] [10.1016/j.watres.2022.119562]

6. Xiang Li, Xianyi Gan.  (2022)  Photo-Fenton degradation of multiple pharmaceuticals at low concentrations via Cu-doped-graphitic carbon nitride (g-C3N4) under simulated solar irradiation at a wide pH range.  Journal of Environmental Chemical Engineering,      [PMID:] [10.1016/j.jece.2022.108290]

7. Jiayi Luo, Yunqiang Yi, Guangguo Ying, Zhanqiang Fang, Yifeng Zhang.  (2021)  Activation of persulfate for highly efficient degradation of metronidazole using Fe(II)-rich potassium doped magnetic biochar.  SCIENCE OF THE TOTAL ENVIRONMENT,      [PMID:34856267] [10.1016/j.scitotenv.2021.152089]

8. Zhenxiao Zheng, Kai Zhu, Zhiyuan Dai.  (2021)  Preparation of Antarctic Krill Oil Emulsion and Its Stability under Catalase Treatment.  Foods,  10  (11): (2797).  [PMID:34829078] [10.3390/foods10112797]

9. Qian Peng, Xuekun Tang, Kun Liu, Xianping Luo, Dongsheng He, Ying Dai, Ganghong Huang.  (2019)  High-Efficiency Catalysis of Peroxymonosulfate by MgO for the Degradation of Organic Pollutants.  Minerals,  10  (1): (2).  [PMID:] [10.3390/min10010002]

10. Shiyu Liu, Rongchang Wang, Cuixiang Ma, Dianhai Yang, Duanxin Li, Zbigniew Lewandowski.  (2019)  Improvement of electrochemical performance via enhanced reactive oxygen species adsorption at ZnO–NiO@rGO carbon felt cathodes in photosynthetic algal microbial fuel cells.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2019.123627]

11. Yunqiang Yi, Guoquan Tu, Pokeung Eric Tsang, Zhanqiang Fang.  (2019)  Insight into the influence of pyrolysis temperature on Fenton-like catalytic performance of magnetic biochar.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2019.122518]

12. Qing-Yuan Li, Xi-Wei Pang, Xing Xing, Xin-Xin Xu, Jun-Jun Jia, Ke-Jiang Zhao, Zhen-Bang Tian, Chao-Jun Wu, Zhong-Liang Zhang, Jing Wang.  (2025)  Facile synthesis of copper sulfide nanoflower decorated amine-grafted polyacrylonitrile fibers for rapid and long-lasting antimicrobial performance mediated by photothermal and photocatalytic effects.  Materials Today Communications,      [PMID:] [10.1016/j.mtcomm.2025.112201]

13. Maochang Xu, Dan Ran, Jian Hu, Jingying Mao, Dehui Qiao, Zongquan Zhang, Xiaoya Liang, Li Zhang, Yu Nie, Hong Yang, Xiangyu Zhou, Chunhong Li.  (2024)  Multifunctional Prussian blue nanozymes alleviate atherosclerosis through inhibiting the inflammation feedback loop.  Journal of Materials Chemistry B,      [PMID:39692245] [10.1039/D4TB01926A]