GRADE & PURITYBioactive?Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure.Recombinant?Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin.ActiBioPure™?ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required.High Performance?High-performance grade with optimized purity and performance characteristics. Use for sensitive analyses where ordinary grades fall short.EnzymoPure™?EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance.≥40U/mg enzyme powder; ≥200U/mg protein
Bioactive,Recombinant,ActiBioPure™,High Performance,EnzymoPure™,≥40U/mg enzyme powder; ≥200U/mg protein ActiBioPure™,Bioactive,High Performance,Recombinant,EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Purine nucleoside phosphorylase (PNP) is a key enzyme in purine metabolism, which is involved in the purine rescue pathway. The deficiency of Purine nucleoside phosphorylase resulted in impaired T cell function. In the presence of inorganic orthophosphate as the second substrate, Purine nucleoside phosphorylase catalyzes the breaking of the glycosidic bond between ribose and deoxyribonucleoside to generate purine base and ribose (deoxyribose) -1-phosphate.
Reaction
Product description
Appearance: White amorphous powder
Source: Microorganism
Enzyme Commission Number: EC 2.4.2.1
CAS Number: 9030-21-1
Storage temperature: -20℃
Activity: ≥40U/mg enzyme powder; ≥200U/mg protein
Unit definition: One unit will cause the phosphorolysis of one micromole of inosine to hypoxanthine and ribose 1-phosphate per min at pH 7.7 at 37℃.
Properties
Stability:
Stable at -20℃ for at least five years
Molecular weight:
32 kDa (SDS-PAGE)
Isoelectric point:
6.0
Michaelis constant:
2.2×10⁻⁴M (Inosine)
Optimum pH:
7.5-8.0
{Fig. 1}
Optimum temperature:
60℃
{Fig. 3}
pH Stability:
6.0-9.0 (30℃, 16hr)
{Fig. 2}
Thermal stability:
< 55℃ (pH 7.7, 30min)
{Fig. 4}
Inhibitors:
Cu²⁺,NEM,Proclin,SDS
Effect of various chemicals:
{Table 1}
Table 1. Effect of Various Chemicals on PNP
[The enzyme dissolved in 50mM K-phosphate buffer, pH 7.5 (10U/ml) was incubated with each chemical at 37℃ for 2hr.]
Specifications
Product Name
Recombinant Purine-Nucleoside Phosphorylase (PNP), CAS No.9030-21-1
ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™
Specifications & Purity
Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥40U/mg enzyme powder; ≥200U/mg protein
Biochemical and Physiological Mechanisms
The purine nucleoside phosphorylases catalyze the phosphorolytic breakdown of the N-glycosidic bond in the beta-(deoxy)ribonucleoside molecules, with the formation of the corresponding free purine bases and pentose-1-phosphate. This protein can degrade al
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