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BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Succinate dehydrogenase (SDH, EC 1.3.5.1) is widely distributed in animals, plants, microorganisms, and cultured cells. As a marker enzyme of mitochondria, it is located on the inner mitochondrial membrane and serves as a crucial link between the respiratory electron transport chain and oxidative phosphorylation. In addition, it also supplies electrons to the energy-generating respiratory chains of various prokaryotic cells. The assay principle of this kit is as follows: SDH catalyzes the dehydrogenation of succinate to fumarate, and the released hydrogen is transferred via phenazine methosulfate (PMS) to reduce 2,6-dichlorophenolindophenol (DCPIP). DCPIP exhibits a characteristic absorption peak at 600 nm. By measuring the rate of change in absorbance at 600 nm, the reduction rate of DCPIP is determined, which allows for the calculation of SDH enzyme activity.
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