BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Room temperature Ships Normal Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Vitamin C, also known as L‑ascorbic acid, is an essential nutrient for higher primates and a few other organisms. It is widely present in various fresh fruits and vegetables. In living organisms, vitamin C is an acidic hexose derivative (an enolic hexose acid lactone) that possesses antioxidant properties, protecting the body from free radical damage, and also functions as a coenzyme. Vitamin C exists in two isomeric forms, the L‑type and the D‑type, but only the L‑type exhibits physiological functions, with both its reduced and oxidized forms being biologically active. The detection principle of this kit is as follows: Under acidic conditions, vitamin C reduces Fe³⁺ to Fe²⁺. The ferrous ion (Fe²⁺) then forms a stable red chelate with phenanthroline, and its absorbance is measured at 534 nm using a microplate reader. Within the concentration range of 0.5–35 μg/mL, the absorbance shows a linear relationship with the vitamin C content, making this method suitable for the determination of vitamin C (ascorbic acid) in plant tissues. The advantages of this method include: a stable reaction with minimal fading, simple operation, excellent specificity (with no interference from reducing sugars or other common reducing substances), and high sensitivity. This kit is for research use only and is not intended for clinical diagnosis or other applications.
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →Our grade selection guide covers purity, stabilizer status, and application suitability for all variants in our catalog.
View BioReagent grade guide →
