Z-D-Arg-Gly-Arg-pNA dihydrochloride (Factor Xa Chromogenic Substrate), CAS No.113711-77-6

CAS: 113711-77-6 Cat. No.: Z962947 Molecular Weight: 641.6823646 PubChem CID: 22874687

GRADE & PURITY BioReagent, ≥98%(HPLC)

Overview

 This product is a colorimetric chromogenic substrate highly specific for Factor Xa (FXa). The sequence Z-D-Arg-Gly-Arg is selectively recognized and cleaved by FXa, releasing p-nitroanilide (pNA), which produces a characteristic yellow absorbance at 405 nm. The change in absorbance is proportional to FXa activity, enabling quantitative measurement.

Molecular Weight: 714.6

Sequence: Z-D-Arg-Gly-Arg-pNA·2HCl

Formula: C₂₈H₄₁Cl₂N₁₁O₇

Key Applications

• Clinical Testing：Anti-FXa activity assay for heparins (unfractionated/LMWH); antithrombin (AT) activity measurement；
• Drug Discovery：Screening and evaluation of FXa inhibitors (e.g., rivaroxaban-type)；
• Basic Research：Coagulation pathway mechanisms; enzyme kinetics (K_m, K_cat) studies；
• Automated Systems：Substrate for coagulation analyzers; raw material for IVD kit development。

 

Specifications

Product Name

Z-D-Arg-Gly-Arg-pNA dihydrochloride (Factor Xa Chromogenic Substrate), CAS No.113711-77-6

Grade

BioReagent

Specifications & Purity

BioReagent, ≥98%(HPLC)

Predicted molecular weight

714.6

CAS

113711-77-6

Molecule Type

Peptide

Storage and Shipping

Storage

Protected from light,Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (12 months). Avoid freeze/thaw cycle. Store in the dark. Desiccated.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Peptides
          • Level6 Oligopeptides

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Peptides
• Direct Parent: Oligopeptides
• Alternative Parents: N-acyl-alpha amino acids and derivatives Alpha amino acid amides Benzyloxycarbonyls Anilides Nitrobenzenes Nitroaromatic compounds N-arylamides N-acyl amines Carbamate esters Secondary carboxylic acid amides Guanidines Propargyl-type 1,3-dipolar organic compounds Organic oxoazanium compounds Carboximidamides Organic zwitterions Carbonyl compounds Organopnictogen compounds Organic oxides Hydrocarbon derivatives Hydrochlorides
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Alpha-oligopeptide - N-acyl-alpha amino acid or derivatives - Alpha-amino acid amide - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - Benzyloxycarbonyl - Nitrobenzene - Anilide - Nitroaromatic compound - N-arylamide - Monocyclic benzene moiety - Benzenoid - N-acyl-amine - Fatty amide - Fatty acyl - Carbamic acid ester - Carboxamide group - Secondary carboxylic acid amide - Guanidine - Organic nitro compound - C-nitro compound - Organic 1,3-dipolar compound - Propargyl-type 1,3-dipolar organic compound - Allyl-type 1,3-dipolar organic compound - Carboximidamide - Organic oxoazanium - Organopnictogen compound - Organic oxygen compound - Organic nitrogen compound - Organic salt - Hydrochloride - Carbonyl group - Hydrocarbon derivative - Organic zwitterion - Organic oxide - Organonitrogen compound - Organooxygen compound - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as oligopeptides. These are organic compounds containing a sequence of between three and ten alpha-amino acids joined by peptide bonds.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y