DNase, RNase free

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DNase, RNase free

Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk.

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  1. Recombinant Uracil-DNA Glycosylase (Heat-labile UDG)
    CAS: 59088-21-0 EC Number: 980-824-3
    Liquid Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 1 U/μL 
    Out of Stock Item #: H292766
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    (psychrophilic marine bacterium) BMTU3346
    Expression system
    E. coli
    Bioactivity
    1 U/μL
    Synonyms
    Uracil DNA Glycosylase | uracil-DNA glycosylase | uracil DNA glycohydrolase | uracil-DNA N-glycosylase | Uracil DNA G...
  2. RNase Inhibitor (Murine, 40U/μL)
    Liquid Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥95%(SDS-PAGE) 40U/μl
    In Stock Item #: R350859
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    Mouse
    Accession #
    Q91VI7
    Expression system
    E. coli
    Bioactivity
    40U/μL
    Synonyms
    Murine Ribonuclease Inhibitor
  3. Recombinant Uracil-DNA Glycosylase (UDG)
    CAS: 59088-21-0 EC Number: 980-824-3
    Liquid Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. 5 U/μL
    Out of Stock Item #: U292571
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    E. coli
    Accession #
    P12295
    Expression system
    E. coli
    Bioactivity
    5 U/μL
    Synonyms
    Uracil DNA Glycosylase | uracil-DNA glycosylase | uracil DNA glycohydrolase | uracil-DNA N-glycosylase | E. coli UDG ...
  4. T4 Polynucleotide Kinase  
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 10 U/μL
    Out of Stock Item #: T665879
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    Pricing & Pack Sizes
    Technical Identifiers
    Synonyms
    T4Polynucleotide Kinase | T4 PNK | Polynucleotide 5'-kinase and 3'-phosphatase | pseT
  5. 2×RNA Loading Buffer
    Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. Suitable for electrophoresis ? Electrophoresis grade — low ionic/UV impurities for clean gel runs. Use in agarose/PAGE electrophoresis where impurities distort bands. for NA electrophoresis ? Nucleic-acid electrophoresis grade — nuclease- and impurity-controlled for DNA/RNA gels. Use to run clean, intact nucleic-acid separations. 2x
    In Stock Item #: R743508
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    Technical Identifiers
    Synonyms
    2×RNA Loading Dye | 2×RNA Gel Loading Buffer
  6. 5' DNA/RNA Adenylase
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 75 U/µL
    Out of Stock Item #: D743509
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    Adenosylase gene of thermophilic bacteria
    Accession #
    8UCH_A,A0A075LQ94
    Expression system
    E. coli
    Bioactivity
    One unit is the amount of enzyme required to catalyze the transfer of 1pmol of AMP to pDNA substrate in a total reaction volume of 20μl in 15 minutes at 65℃.
    Synonyms
    Single-Strand Nucleic Acid 5' Adenylase
  7. Biotin-11-dUTP (1 mM, Nuclease free)
    CAS: 86303-25-5 (free acid) Formula: C28H45N6O17P3S (free acid) Molecular Weight: 862.68 (free acid)
    Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥95% 1 mM
    Out of Stock Item #: B744151
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    Pricing & Pack Sizes
    Technical Identifiers
    Synonyms
    Biotin-11-dUTP | Biotin-X-(5-aminoallyl)-dUTP | Biotin-11-2'-deoxyuridine-5'-triphosphate
  8. BBiotin-16-UTP (10mM)
    Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. PCR Reagent ? PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. For In Vitro Transcription ? In-vitro transcription grade — nuclease-free reagents for RNA synthesis in vitro. Use to make RNA transcripts/probes free of degradation. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥95% 10mM
    In Stock Item #: B744152
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    Pricing & Pack Sizes
    Technical Identifiers
    Synonyms
    Biotin-16-UTP | Biotin-16-(5-aminoallyl)-UTP;Biotin-16-Aminoallyluridine-5'-Triphosphate | Biotinyl-ε-aminocaproyl-γ-...
  9. Bst DNA Polymerase, Large Fragment
    Liquid Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 8.0 U/μL
    Out of Stock Item #: B744266
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    Geobacillus stearothermophilus
    Accession #
    Q45458
    Expression system
    E. coli
    Bioactivity
    One unit is defined as the amount of enzyme that will incorporate 10nmol of dNTP into acid insoluble material in 30 minutes at 65℃.
    Synonyms
    (Bacillus stearothermophilus, Bst) DNA Polymerase I , Large Fragment | DNA polymerase I | polA
  10. Terminal Deoxynucleotidyl Transferase (TdT)
    Liquid Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 5.0 U/μL
    Out of Stock Item #: T751007
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    Pricing & Pack Sizes
    Technical Identifiers
    Species
    Bovine
    Accession #
    P06526
    Expression system
    E. coli
    Bioactivity
    One Unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
    Synonyms
    TdT | DNTT | ‌ DNA nucleotidylexotransferase‌‌ | Terminal addition enzyme‌‌ | Terminal deoxynucleotidyltransferase‌‌ ...
  11. Terminal Deoxynucleotidyl Transferase (TdT)
    CAS: 9027-67-2 EC Number: 232-819-6
    Liquid Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. 20 U/μL
    Out of Stock Item #: T751008
    View Product
    Pricing & Pack Sizes
    Technical Identifiers
    Species
    Bovine
    Accession #
    P06526
    Expression system
    E. coli
    Bioactivity
    One unit is the amount of enzyme required to catalyze the incorporation of 1 nmol of dNTP to the 3'-OH termini of a polynucleotide within 60 minutes at 37℃.
    Synonyms
    TdT | DNTT | ‌ DNA nucleotidylexotransferase‌‌ | Terminal addition enzyme‌‌ | Terminal deoxynucleotidyltransferase‌‌ ...
  12. Thermostable RNase H
    Liquid Suitable for molecular biology ? Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents. BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. DNase, RNase free ? Free of DNase and RNase activity to keep DNA and RNA intact. Use in nucleic-acid extraction, PCR, and storage where degradation is a risk. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. Sterile ? Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization. for DNA and RNA applications ? For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk. ≥95% 5 U/μl
    Out of Stock Item #: T751065
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    Pricing & Pack Sizes
    Technical Identifiers
    Synonyms
    Thermostable RNase H | RNase H
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Frequently Asked Questions — DNase, RNase free

What documentation is provided? +

Certificate of Analysis with QC test results, SDS, and where applicable lot-specific functional or activity data. Application notes and protocol guidance are available on the product page.

What does DNase, RNase free mean? +

DNase, RNase free indicates that the product has been processed and tested for sensitive nucleic acid and protein workflows. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.

Is DNase, RNase free certified DNase / RNase / protease free? +

Many molecular biology grades include nuclease activity testing. The exact tests and limits are listed on the Certificate of Analysis. For applications sensitive to nuclease contamination (qPCR, NGS library prep, mRNA work), prefer grades explicitly labeled DNase free, RNase free or Protease Free.

Can I use DNase, RNase free for sensitive workflows like qPCR or NGS? +

Yes, where the product is qualified for the workflow. For PCR / qPCR, look for For PCR or PCR Reagent grade. For NGS sample prep, prefer molecular biology, RNase free or BioReagent grade — and verify lot QC data.

How does DNase, RNase free differ from analytical reagent grades? +

Analytical grades focus on chemical purity. Molecular biology grades add tests directly relevant to nucleic-acid and protein workflows — nuclease activity, microbial limits, sometimes endotoxin. Use molecular-biology grades whenever a downstream enzyme or biological readout is involved.

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