for Northern and Southern blotting
Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting.
Popular Products
Denhardt′s Solution 50xLiquid for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. solutionIn Stock Item #: D485245View ProductPricing & Pack SizesTechnical Identifiers
- Synonyms
- blocking reagent | blocking solution | denhardts | hybridization buffer | hybridization solution | northern blocking ...
- Nitrocellulose Membrane (NC)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.45 μm; 300x3000mmOut of Stock Item #: N766354View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- Nitrocellulose transfer membrane
- Nitrocellulose Membrane (NC)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.22 μm; 300x3000mmOut of Stock Item #: N766340View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- Nitrocellulose membrane
- Polyvinylidene fluoride Membrane (PVDF)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.45 μm; 300x3000mmOut of Stock Item #: P766361View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- Polyvinylidene Fluoride | PVDF | Polyvinylidene fluoride PVDF transfer Membrane
- Nitrocellulose Membrane (NC)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.22 μm; 100x100mmOut of Stock Item #: N766329View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- Nitrocellulose transfer membrane
- Nitrocellulose Membrane (NC)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.45 (μm), size: 100x100mmOut of Stock Item #: N766351View ProductPricing & Pack Sizes
Technical Identifiers
- Synonyms
- Nitrocellulose transfer membrane
- Polyvinylidene fluoride Membrane (PVDF)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.22 (μm), size: 100x100mmOut of Stock Item #: P766356View ProductPricing & Pack Sizes
Technical Identifiers
- Polyvinylidene fluoride Membrane (PVDF)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.45μm; 100x100mmOut of Stock Item #: P766360View ProductPricing & Pack Sizes
Technical Identifiers
- Polyvinylidene fluoride Membrane (PVDF)BioReagent ? BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility. for Northern and Southern blotting ? Northern/Southern blot grade — nuclease-controlled for RNA/DNA blot transfer. Use to keep nucleic acids intact through blotting. for Western blot ? Western-blot grade — suited to protein transfer and immunodetection. Use in Western workflows where reagent purity keeps background low. Aperture: 0.22 μm; 300x3000mmOut of Stock Item #: P766358View ProductPricing & Pack Sizes
Technical Identifiers
Frequently Asked Questions — for Northern and Southern blotting
What does for Northern and Southern blotting mean?
for Northern and Southern blotting indicates that the product has been processed and tested for electrophoresis and blotting. Typical specifications include low or undetectable nuclease/protease activity, low endotoxin, controlled microbial limits and high lot-to-lot reproducibility.
Is for Northern and Southern blotting certified DNase / RNase / protease free?
Many molecular biology grades include nuclease activity testing. The exact tests and limits are listed on the Certificate of Analysis. For applications sensitive to nuclease contamination (qPCR, NGS library prep, mRNA work), prefer grades explicitly labeled DNase free, RNase free or Protease Free.
Can I use for Northern and Southern blotting for sensitive workflows like qPCR or NGS?
Yes, where the product is qualified for the workflow. For PCR / qPCR, look for For PCR or PCR Reagent grade. For NGS sample prep, prefer molecular biology, RNase free or BioReagent grade — and verify lot QC data.
How does for Northern and Southern blotting differ from analytical reagent grades?
Analytical grades focus on chemical purity. Molecular biology grades add tests directly relevant to nucleic-acid and protein workflows — nuclease activity, microbial limits, sometimes endotoxin. Use molecular-biology grades whenever a downstream enzyme or biological readout is involved.
What documentation is provided?
Certificate of Analysis with QC test results, SDS, and where applicable lot-specific functional or activity data. Application notes and protocol guidance are available on the product page.