Fast Blue BB salt - Dye content ≥80 % , CAS No.5486-84-0

CAS: 5486-84-0 Cat. No.: F106955 Molecular Weight: 415.94 Beilstein Registry Number: 1845288 EC Number: 226-817-4

GRADE & PURITY Dye content ≥80 %

Synonyms

FAST BLUE BB SALT | Fast Blue BB Salt hemi(zinc chloride) salt | Fast Blue BB Salt hemi(zinc chloride) salt, for microscopy (Hist.) | Benzenediazonium, 4-(benzoylamino)-2,5-diethoxy-, (T-4)-tetrachlorozincate(2-) (2:1) | Fast Blue BB Salt hemi(zinc chlori

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Fast Blue BB Salt hemi(zinc chloride) salt, also known as Cl Azoic diazo 20 (Cl 37175) is a diazonium salt that possesses a lipophilic amine lipophilic with an aromatic system of moderate size. It is commercially available as the zinc chloride double salt.

Application

Fast blue BB salt is suitable for the staining of enterochromaffin. Its applications are seen in enzyme histochemistry by Gomori and are suitable for the histochemical demonstration of beta-glucuronidase activities, and various peptidases and proteases. It is also used in enzyme histochemistry including the demonstration of serine esterase in blood smears and histological sections. Fast blue BB salt plays a vital role as a routine stain to demonstrate alkaline phosphatase labeled antibodies.

Fast Blue BB Salt hemi(zinc chloride) salt has been used for alkaline phosphatase staining in cells.

Specifications

Synonyms

FAST BLUE BB SALT | Fast Blue BB Salt hemi(zinc chloride) salt | Fast Blue BB Salt hemi(zinc chloride) salt, for microscopy (Hist.) | Benzenediazonium, 4-(benzoylamino)-2, 5-diethoxy-, (T-4)-tetrachlorozincate(2-) (2:1) | Fast Blue BB Salt hemi(zinc chlori

Specifications & Purity

Dye content ≥80 %

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Pubchem Sid: 504768200
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504768200
• Canonical Smiles: CCOC1=CC(=C(C=C1NC(=O)C2=CC=CC=C2)OCC)[N+]#N.CCOC1=CC(=C(C=C1NC(=O)C2=CC=CC=C2)OCC)[N+]#N.[Cl-].[Cl-].Cl[Zn]Cl
• IUPAC Name: 4-benzamido-2,5-diethoxybenzenediazonium;dichlorozinc;dichloride
• InChIKey: CMFRFQODFZBKTI-UHFFFAOYSA-L
• INCHI: 1S/2C17H17N3O3.4ClH.Zn/c2*1-3-22-15-11-14(20-18)16(23-4-2)10-13(15)19-17(21)12-8-6-5-7-9-12;;;;;/h2*5-11H,3-4H2,1-2H3;4*1H;/q;;;;;;+2/p-2
• Isomeric SMILES: CCOC1=CC(=C(C=C1NC(=O)C2=CC=CC=C2)OCC)[N+]#N.CCOC1=CC(=C(C=C1NC(=O)C2=CC=CC=C2)OCC)[N+]#N.[Cl-].[Cl-].Cl[Zn]Cl
• WGK Germany: 3
• UN Number: 2811
• Molecular Weight: 415.94
• Beilstein: 1845288

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Class Benzene and substituted derivatives
      • Subclass Anilides
        • Level5 Aromatic anilides
          • Level6 Benzanilides

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Class: Benzene and substituted derivatives
• Subclass: Anilides
• Intermediate Tree Nodes: Aromatic anilides
• Direct Parent: Benzanilides
• Alternative Parents: Benzamides Phenoxy compounds Phenol ethers Benzoyl derivatives Alkyl aryl ethers Secondary carboxylic acid amides Organic diazonium salts Organic transition metal salts Organic oxides Hydrocarbon derivatives Organic cations
• Molecular Framework: Not available
• Substituents: Benzanilide - Benzamide - Benzoic acid or derivatives - Phenoxy compound - Benzoyl - Phenol ether - Alkyl aryl ether - Carboxamide group - Organic diazonium salt - Secondary carboxylic acid amide - Organic metal salt - Ether - Carboxylic acid derivative - Organic transition metal salt - Organic oxygen compound - Organooxygen compound - Organonitrogen compound - Organic nitrogen compound - Hydrocarbon derivative - Organic oxide - Organic salt - Organic cation - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as benzanilides. These are aromatic compounds containing an anilide group in which the carboxamide group is substituted with a benzene ring. They have the general structure RNC(=O)R', where R,R'= benzene.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: Soluble in water.
• Sensitivity: Light sensitive.
• Melt Point(°C): 157°C
• Molecular Weight: 831.900 g/mol
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 10
• Rotatable Bond Count: 12
• Exact Mass: 830.071 Da
• Monoisotopic Mass: 828.074 Da
• Topological Polar Surface Area: 151.000 Ų
• Heavy Atom Count: 51
• Formal Charge: 0
• Complexity: 431.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 5

XLogP3

References

1. Haiqian Zhao, Jihui Gao, Wei Zhou, Zhonghua Wang, Shaohua Wu.  (2015)  Quantitative detection of hydroxyl radicals in Fenton system by UV-vis spectrophotometry.  Analytical Methods,  7  (13): (5447-5453).  [PMID:] [10.1039/C5AY00514K]

2. Zhen Wang, Lei Ying, Weibin Xu, Beien Zhu, Chuande Huang, Pengfei Wu, Qingqing Gu, Bing Yang, Jian Lin, Yi Gao, Xiaodong Wang, Tao Zhang.  (2025)  Aunano-Fe1 tandem catalysis for promoted methane conversion to acetic acid by O2 oxidation.  APPLIED CATALYSIS B-ENVIRONMENTAL,      [PMID:] [10.1016/j.apcatb.2025.125632]