BioReagent BioReagent for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Cholesterol, also known as cholesterin, is a derivative of cyclopentanoperhydrophenanthrene. It is widely distributed in animals, with the highest concentrations found in the brain and nerve tissues. It is also relatively abundant in the kidneys, spleen, skin, liver, and bile. The enzymatic method for determining Free Cholesterol (FC) is a common technique in biochemical assays. In blood, approximately one-third of cholesterol exists as free cholesterol, while the remaining two-thirds is in the form of cholesterol esters bound to fatty acids. These cholesterol esters are hydrolyzed into free cholesterol by cholesterol esterase (CEH). The free cholesterol is then oxidized by cholesterol oxidase (COD) to produce cholesterone and hydrogen peroxide. Subsequently, in the presence of peroxidase (POD), the hydrogen peroxide catalyzes the reaction between 4-aminoantipyrine and phenol (collectively referred to as PAP), generating a red quinoneimine dye (Trinder reaction). When CEH is absent, cholesterol esters cannot be hydrolyzed, allowing for the measurement of free cholesterol content only. Absorbance is measured using a spectrophotometer at wavelengths between 500 nm and 520 nm. This assay kit is primarily designed for the quantitative determination of free cholesterol in samples such as human or animal serum, plasma, cerebrospinal fluid, cells, and tissues. It is intended for research use only and is not suitable for clinical diagnosis or other purposes.
Comprehensive hazard, handling, storage, and regulatory compliance document.
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