Glutamate Oxidase (GLOD), CAS No.39346-34-4

CAS: 39346-34-4 Cat. No.: G774083

GRADE & PURITY Bioactive, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥40 U/mg protein

Accession #

Q8L3C7

Expression system

E. coli

Bioactivity

≥40 U/mg protein; Unit definition: One unit will convert one micromole of L-glutamate to α- ketoglutarate per min at pH 6.5 at 37℃.

Overview

Source: Microorganism
Isoelectric point: 5.6
Michaelis constant: 2.8×10^-4 M (L-Glutamate)
Optimum pH: 6.0~8.0                     Fig. 1
Optimum temperature: 50~65℃                     Fig. 3
pH Stability: 4.0~7.5 (25℃, 24hr)                     Fig. 2
Thermal stability: <45℃ (pH 6.5, 30min)                     Fig. 4
Inhibitors: Ca2+, Co2+, Cu2+, Fe3+, Mn2+, K4Fe(CN)6, Proclin, SDS
Effect of various chemicals:                     Table 1
Reaction：

Specifications

Product Name

Glutamate Oxidase (GLOD), CAS No.39346-34-4

Synonyms

L-Glutamate oxidase | LGOX

Grade

ActiBioPure™, Bioactive, High Performance, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, EnzymoPure™, High Performance, ≥90%(SDS-PAGE), ≥40 U/mg protein

Bioactivity

≥40 U/mg protein; Unit definition: One unit will convert one micromole of L-glutamate to α- ketoglutarate per min at pH 6.5 at 37℃.

Accession #

Q8L3C7

SDS-PAGE

77 kDa (SDS-PAGE)

CAS

39346-34-4

Enzyme Commission Number

EC 1.4.3.11

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥40 U/mg protein

Storage

Protected from light,Store at -20°C,Desiccated,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at 4℃ short term (15 days). Store at -20℃ long term (2 years). Upon delivery aliquot. Avoid freeze/thaw cycle. Store in the dark. Desiccated