HPF - Moligand™, ≥98%, A solution in methyl acetate , CAS No.359010-69-8

CAS: 359010-69-8 Cat. No.: R336295 Formula: C₂₆H₁₆O₆ Molecular Weight: 424.40 PubChem CID: 16760638

GRADE & PURITY Moligand™, ≥98%, A solution in methyl acetate

Synonyms

Hydroxyphenyl Fluorescein | 3'-hydroxy-6'-(4-hydroxyphenoxy)spiro[2-benzofuran-3,9'-xanthene]-1-one | 2-[6-(4-Hydroxyphenoxy)-3-oxo-3H-xanthen-9-yl]benzoic acid

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

ROS Probe, HPF is a cell-permeable, photo-stable hydroxyphenyl-substituted fluorescein compound that acts as a highly sensitive fluorescent probe for the detection of hydroxyl radical and peroxynitrite. The compound has little activity towards other reactive oxygen species (ROS), such as hypochlorite, singlet oxygen, superoxide, hydrogen peroxide, nitric oxide and alkyl peroxide.

Purpose

Hydroxyphenyl fluorescein (HPF) is the reagent that can directly detect highly reactive oxygen species (hROS). Hydroxyphenyl fluorescein selectively and dose-dependently reacts with hROS, such as the hydroxyl radical and peroxynitrite, which exhibit strong fluorescence 

Specifications

Synonyms

Hydroxyphenyl Fluorescein | 3'-hydroxy-6'-(4-hydroxyphenoxy)spiro[2-benzofuran-3,9'-xanthene]-1-one | 2-[6-(4-Hydroxyphenoxy)-3-oxo-3H-xanthen-9-yl]benzoic acid

Specifications & Purity

Moligand™, ≥98%, A solution in methyl acetate

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Purity

≥98%

Product Properties

Excitation and Emision Ranges

λex ~490 nm, λem ~515 nm

Names and Identifiers

Pubchem Sid

528764816

Canonical Smiles

C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)OC6=CC=C(C=C6)O

IUPAC Name

3'-hydroxy-6'-(4-hydroxyphenoxy)spiro[2-benzofuran-3,9'-xanthene]-1-one

InChIKey

NPOSMYDPUKBGQL-UHFFFAOYSA-N

INCHI

1S/C26H16O6/c27-15-5-8-17(9-6-15)30-18-10-12-22-24(14-18)31-23-13-16(28)7-11-21(23)26(22)20-4-2-1-3-19(20)25(29)32-26/h1-14,27-28H

Isomeric SMILES

C1=CC=C2C(=C1)C(=O)OC23C4=C(C=C(C=C4)O)OC5=C3C=CC(=C5)OC6=CC=C(C=C6)O

PubChem CID

16760638

UN Number

2265

Packing Group

III

Molecular Weight

424.40

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organoheterocyclic compounds
    • Class Benzopyrans
      • Subclass 1-benzopyrans
        • Level5 Dibenzopyrans
          • Level6 Xanthenes

• Kingdom: Organic compounds
• Superclass: Organoheterocyclic compounds
• Class: Benzopyrans
• Subclass: 1-benzopyrans
• Intermediate Tree Nodes: Dibenzopyrans
• Direct Parent: Xanthenes
• Alternative Parents: Diarylethers Phthalides Benzofuranones Isobenzofurans Phenoxy compounds Phenol ethers 1-hydroxy-2-unsubstituted benzenoids Lactones Carboxylic acid esters Oxacyclic compounds Organic oxides Hydrocarbon derivatives
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Xanthene - Diaryl ether - Benzofuranone - Phthalide - Isobenzofuranone - Isocoumaran - Isobenzofuran - Phenoxy compound - Phenol ether - Phenol - 1-hydroxy-2-unsubstituted benzenoid - Benzenoid - Monocyclic benzene moiety - Lactone - Carboxylic acid ester - Oxacycle - Ether - Carboxylic acid derivative - Organooxygen compound - Hydrocarbon derivative - Organic oxygen compound - Organic oxide - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as xanthenes. These are polycyclic aromatic compounds containing a xanthene moiety, which consists of two benzene rings joined to each other by a pyran ring.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Solubility: ≤20mg/ml in ethanol;20mg/ml in DMSO;20mg/ml in dimethyl formamide
• Sensitivity: Light sensitive
• Flash Point(°F): 8.6°F
• Flash Point(°C): -13℃
• Boil Point(°C): 57℃（134.6°F）
• Molecular Weight: 424.400 g/mol
• XLogP3: 5.000
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 6
• Rotatable Bond Count: 2
• Exact Mass: 424.095 Da
• Monoisotopic Mass: 424.095 Da
• Topological Polar Surface Area: 85.200 Ų
• Heavy Atom Count: 32
• Formal Charge: 0
• Complexity: 704.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 1
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1