Mouse Beta-1,4-galactosyltransferase 1 (Y286L)

Cat. No.: M1507860

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, His Tag, >2000 U/mg protein；Protein concentration: See COA; expressed in HEK293

Accession #

P15535

Protein Tag

C-His

Expression system

HEK293

Bioactivity

>2000 U/mg protein

Overview

  Mouse Beta-1,4-galactosyltransferase 1 (Y286L) is a β1,4-N-acetylgalactosaminyltransferase that can efficiently transfer GalNAc or its derivatives such as GalNAz to terminal GlcNAc residues. The latter can be applied to antibody-drug conjugates (ADCs) for targeted cancer therapy and other related applications.

Reaction Formula

Product Properties

Source: HEK293

Label: C-terminal His Tagged

Molecular weight: 50.1 kDa

Storage Buffer: 20 mM Tris-HCl, 200 mM NaCl, 20% Glycerin, pH 7.4

Enzyme activity: >2000 U/mg protein

Unit Definition: The amount of enzyme (mg) required to transfer 1 nmol of GalNAz to GlcNAc per minute at 37℃.

Transport and Storage Methods

Transported on ice packs or dry ice; stored at -20℃; valid for 18 months.

Recommended Usage

1.Reaction System: 50 mM Tris-HCl (pH 7.5), 6 mM MnCl₂

2.Reaction Reagents: ① UDP-GalNAz:antibody (equivalent ratio) = 300:1; ② Enzyme addition amount: enzyme/antibody = 5% (mass ratio)

3.Prepare the reaction solution according to the above conditions, incubate at 37℃ for 48 hours; then place at -15 to -25℃ to terminate the reaction and conduct sample detection.

Precautions

Please pay attention to experimental safety and operate with gloves. This product is for research use only!

Specifications

Product Name

Mouse Beta-1,4-galactosyltransferase 1 (Y286L)

Synonyms

Mouse B4GalT1 (Y286L) | B4GALT1 | B4galt | GalT | Ggtb | Ggtb2 | b4Gal-T1 | B-1, 4-GalT | B-1, 4-GalT1 | beta4Gal-T1 | beta-1, 4-GalT | beta-1, 4-GalT1

Grade

ActiBioPure™, Bioactive, High Performance, His Tag, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, His-Tag, >2000 U/mg protein；Protein concentration: See COA; expressed in HEK293

Biochemical and Physiological Mechanisms

Beta-1, 4-galactosyltransferase 1: The Golgi complex form catalyzes the production of lactose in the lactating mammary gland and could also be responsible for the synthesis of complex-type N-linked oligosaccharides in many glycoproteins as well as the carb

Bioactivity

>2000 U/mg protein

Accession #

P15535

Predicted molecular weight

50.1 kDa

Enzyme Commission Number

EC 2.4.1-

Molecule Type

Enzyme

Storage and Shipping

Concentration

>2000 U/mg protein；Protein concentration: See COA; expressed in HEK293

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (18 months). Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.

Unit definition

The amount of enzyme (mg) required to transfer 1 nmol of GalNAz to GlcNAc per minute at 37℃.