NGS TP Index Kit for Illumina (Index Primers Set II) - 384 rxns, high purity

Cat. No.: N665978

Overview

Products content

N665978	Component	384 T	Storage
N665978A	Index N502-N522 Primers for Illumina	16×24 μL	-20℃. Avoid freeze/thaw cycle.
N665978B	Index N701-N729 Primers for Illumina	24×16 μL	-20℃. Avoid freeze/thaw cycle.

Products Introduction

This kit is a companion to the transposase-based rapid DNA library construction kit, designed for Illumina platform library construction. It contains 16 N5 primers and 24 N7 primers, which can be used to prepare 384 different bipartite Index libraries. All reagents provided in the kit have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The libraries can be used for sequencing on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq.

Provide your own instruments, reagents and consumables

1. Magnetic frame: DynaMagTM-2 is recommended.

2. DNA purification and recovery kit: It is recommended to use Kangwei DNA purification and recovery kit by magnetic bead method.

3. DNA building kit: It is recommended to use the Kangwei Century transposase method second-generation sequencing rapid DNA building kit.

4. Anhydrous ethanol.

5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;

Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.

Pre-experiment Preparation and Important Notes

Please centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.

procedure

For the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.

Index N502-N522 Primers for Illumina

 

Index N701-N729 Primers for Illumina

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads