NGS TP Index Kit for Illumina (Index Primers Set VI) (copy) - 240 rxns, high purity

Cat. No.: N669983

GRADE & PURITY 240 rxns

Overview

Products content

N669983	Component	240 T	Storage
N669983A	Index N501 Primers for Illumina	240 μL	-20℃. Avoid freeze/thaw cycle.
N669983B	Index N973-N996 Primers for Illumina	24×10 μL	-20℃. Avoid freeze/thaw cycle.

Products Introduction

This kit is a companion kit to the transposase-based Rapid DNA Library Construction Kit for Illumina platform library construction. Each kit contains one N5 primer and 24 N7 primers, which can be used to prepare 24 different single-ended Index libraries. All reagents provided in the kits have been subjected to stringent quality control and functional validation to maximize the stability and reproducibility of library construction. The libraries can be used for sequencing on Illumina platforms such as HiSeq X-10/4000/2500/2000 and MiSeq.

Provide your own instruments, reagents and consumables

1. Magnetic frame: DynaMagTM-2 is recommended.

2. DNA purification and recovery kit: It is recommended to use  DNA purification and recovery kit by magnetic bead method.

3. DNA building kit: It is recommended to use the  Century transposase method second-generation sequencing rapid DNA building kit.

4. Anhydrous ethanol.

5. Reaction tubes: It is recommended to use low adsorption PCR tubes with 1.5 ml centrifuge tubes;

Tip: It is recommended to use a high quality filter tip to prevent contamination of kits and library samples.

Pre-experiment Preparation and Important Notes

Please centrifuge briefly before opening the cap so that the liquid collects at the bottom of the tube to avoid cross-contamination between different primers.

Procedure

For the use of the CombiVision Second Generation Sequencing Multisample Primer Kit, please follow the CombiVision Second Generation Sequencing Rapid DNA Library Kit protocol.

Index N501 Primer for Illumina

Index N901-N996 Primer for Illumina

Storage and Shipping

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads