o-Phenylenediamine dihydrochloride - tablet, 60 mg substrate per tablet , CAS No.615-28-1

CAS: 615-28-1 Cat. No.: O755378 Molecular Weight: 181.06 Beilstein Registry Number: 3912045 EC Number: 210-418-7

GRADE & PURITY tablet, 60 mg substrate per tablet

Storage

Store at 2-8°C

Shipped In

Wet ice,FedEx DG Service

Overview

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

o-Phenylenediamine dihydrochloride (OPD) is a chromogenic substrate that can be used in enzyme-linked immunosorbent assay (ELISA) procedures as it utilizes horseradish peroxidase conjugates. The substrate produces a soluble end product that is orange-brown and can be read spectrophotometrically at 450 nm. The OPD reaction may be stopped with 3N HCl or 3M H2SO4 and read at 492 nm.

Application:

o-Phenylenediamine dihydrochloride has been used as a stop solution for evaluating bovine serum albumin (BSA) antibodies via enzyme-linked immunosorbent assay (ELISA). It has also been used to measure specific antibodies in pig serum by ELISA.

Specifications

Specifications & Purity

tablet, 60 mg substrate per tablet

Storage

Store at 2-8°C

Shipped In

Wet ice, FedEx DG Service

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Canonical Smiles: C1=CC=C(C(=C1)N)N.Cl.Cl
• IUPAC Name: benzene-1,2-diamine;dihydrochloride
• InChIKey: RIIWUGSYXOBDMC-UHFFFAOYSA-N
• INCHI: 1S/C6H8N2.2ClH/c7-5-3-1-2-4-6(5)8;;/h1-4H,7-8H2;2*1H
• Isomeric SMILES: C1=CC=C(C(=C1)N)N.Cl.Cl
• WGK Germany: 3
• RTECS: ST0175000
• UN Number: 2811
• Packing Group: I
• Molecular Weight: 181.06
• Beilstein: 3912045
• Reaxy-Rn: 3912045

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Benzenoids
    • Class Benzene and substituted derivatives
      • Subclass Aniline and substituted anilines

• Kingdom: Organic compounds
• Superclass: Benzenoids
• Class: Benzene and substituted derivatives
• Subclass: Aniline and substituted anilines
• Intermediate Tree Nodes: Not available
• Direct Parent: Aniline and substituted anilines
• Alternative Parents: Primary amines Organopnictogen compounds Hydrochlorides Hydrocarbon derivatives
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Aniline or substituted anilines - Organic nitrogen compound - Organopnictogen compound - Hydrocarbon derivative - Hydrochloride - Primary amine - Organonitrogen compound - Amine - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as aniline and substituted anilines. These are organic compounds containing an aminobenzene moiety.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Melt Point(°C): 205°C(dec.)(lit.)
• Molecular Weight: 181.060 g/mol
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 2
• Rotatable Bond Count: 0
• Exact Mass: 180.022 Da
• Monoisotopic Mass: 180.022 Da
• Topological Polar Surface Area: 52.000 Ų
• Heavy Atom Count: 10
• Formal Charge: 0
• Complexity: 62.900
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 3

XLogP3

References

1. Lu Sun, Qian-Yue Xu, Hai-Bo Wang.  (2025)  A magnetic separated-type ultrasensitive fluorescent DNA biosensor based on hydrogen peroxide triggered chemically driven redox-cycling system.  NEW JOURNAL OF CHEMISTRY,      [PMID:] [10.1039/D5NJ02324F]

2. Linglin Fu, Yifan Qian, Jinru Zhou, Huan Li, Yanbo Wang.  (2019)  Quantification of shellfish Arginine kinases by double-enhanced immunoassay employing magnetic beads and gold nanoparticles as carrier.  LWT-FOOD SCIENCE AND TECHNOLOGY,      [PMID:] [10.1016/j.lwt.2019.108916]