Phaseolus vulgaris lectin P (PHA-P), native isomer composition

Cat. No.: P419332

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Features 

Ultrapure quality

Agglutinates leucocytes and erythrocytes

Mixture of L and E subunits

Sugar specificity: complex oligosaccharides

Lyophilized powder

Product description

Phaseolus vulgaris lectin P or Phytohemagglutinin (PHA-P)is isolated from red kidney beans and purified by affinitychromatography. PHA-P is a mixture of isolectins containingL (leucoagglutinin) and E (erythroagglutinin) subunits incompositions L1E3, L2E2, L3E1, E4.

Thus PHA-P is the form of PHA before separation oferythroagglutinin and leucoagglutinin. PHA-P has amolecular weight of 128 kDa and specificity for complexoligosaccharides . PHA-P is not blood group specific and agglutinates human erythrocytes blood group 0 with alectin concentration of ≤4 μg/ml.

PHA-P lectin is supplied as a white to light-pinkessentially salt-free lyophilized powder from 50 mM NH4HCO3,which evaporates during the lyophilization procedure. Thepurity of the lectin is determined by SDS-PAGE generatingone single band.

Applications

Lateral flow erythrocyte separation

Typical subunit composition

Total L subunit composition: 60-65%

Total E subunit composition: 35-40%

L4 25-35%

L3E1 20-30%

L2E2 10-20%

L1E3 17-27%

E4 1-11%

Directions for use

The lectin may be reconstituted in a suitable buffer for yourapplication.

Shipping and storage

The product is shipped at -20°C, however for over-the-daytransport it may be shipped at ambient temperature. Thelyophilized powder is stable for more than five years fromproduction date when stored below -20°C. After reconstitutionwith PBS, pH 7.4, the solution may be stored frozen in workingaliquots for up to 12 months.

Specifications

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.