Phosphatase, Acid from wheat germ(Triticum vulgare), CAS No.9001-77-8

CAS: 9001-77-8 Cat. No.: P128633 Molecular Weight: 114.14 EC Number: 232-630-9 PubChem CID: 12951370

GRADE & PURITY Bioactive, ActiBioPure™, Native, High Performance, EnzymoPure™, ≥0.15 units/mg dry weight

Bioactivity

≥0.15 U/mg dry weight

Overview

Acid phosphatase is an esterase with broad activity at an optimal pH below 7.0. There are three isozymes, EI, EII, and EIII of similar molecular weight (55 kDa± 5 kDa). Their optimum pH's are 5.5, 4.5, and 4.0 respectively.

Acid phosphatase is ubiquitous in nature, perhaps one of the most concentrated sources being human prostate gland, a fact exploited by the clinical chemist who measures the serum enzyme level as an index of prostatic cancer. It has also been associated with Gaucher's disease, patients exhibiting unique peaks in their electrophoresed sera. Acid phosphatases have been reviewed by Hollander (1971) although the wheat germ enzyme is not included.

Acid phosphatase activity was observed by Teller in 1954 in preparations of a wheat germ lipase described by Singer in 1948. Subsequent work confirmed that the non-specific esterase activity of the wheat germ preparation may be measured both as lipase (tracetin as substrate) and phosphatase.

Acid phosphatase catalyzes the following reaction at an optimal pH below 7:

Orthophosphoric monoester + H₂O → alcohol + H₃PO₄

Characteristics of Acid Phosphatase from Wheat Germ:

Specificity: The enzyme has a broad esterase activity. It shows highest activity for pyrophosphate.

Composition: Three isozymes of closely similar molecular weights: EI, EII, and EIII.

Characteristics of Phosphatase, Acid

Molecular Weight: 55,000 ± 5,000.

Optimal pH: EI - 5.5, EII - 4.5, and EIII - 4.0.

Inhibitors: Fluoride, molybdate and orthophosphate.

Specifications

Product Name

Phosphatase, Acid from wheat germ(Triticum vulgare), CAS No.9001-77-8

Synonyms

3-(2-propynyloxy)propan-2-ol | SCHEMBL4278701 | 1-(PROP-2-YN-1-YLOXY)PROPAN-2-OL | DTXSID701009098 | 1-prop-2-ynoxypropan-2-ol | 1-Prop-2-inyl-naphthalin | Acidphosphatase | 1-(prop-2-ynyloxy)propan-2-ol | Phosphatase, acid | AKOS015917690 | 9001-77-8 | N

Grade

ActiBioPure™, Bioactive, High Performance, Native, EnzymoPure™

Specifications & Purity

Bioactive, ActiBioPure™, High Performance, EnzymoPure™, Native, ≥0.15 units/mg dry weight

Biochemical and Physiological Mechanisms

Plant acid phosphatase (APase) is localized in cytosol, vacuoles, and cell walls. Plant acid phosphatase is important for phosphate acquisition to mobilize organic phosphates in the soil. Phosphate starvation induces acid phosphatase generation. Acid phos

Bioactivity

≥0.15 U/mg dry weight

CAS

9001-77-8

Enzyme Commission Number

3.1.3.2

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥0.15 units/mg dry weight

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

One Unit hydrolyzes one micromole of o-carboxyphenyl phosphate per minute at 25°C, pH 5.0.

References

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