PNGase F (MS), CAS No.83534-39-8

CAS: 83534-39-8 Cat. No.: rp227283 EC Number: 964-131-3
AVAILABLE TO ORDER
GRADE & PURITY Animal Free ? Animal-free — produced without animal-derived components to reduce contamination risk. Use in biomanufacturing and culture avoiding animal-origin material. Carrier Free ? Carrier-free — supplied without added carrier protein/stabilizer. Use when carriers (e.g. BSA) would interfere with conjugation or sensitive assays. Bioactive ? Bioactive grade — verified to retain biological activity in functional assays. Use when the molecule must be functionally active, not just pure. Recombinant ? Recombinant — produced via recombinant expression for defined sequence and consistency. Use for reproducible, animal-free proteins of known origin. Suitable for mass spectrometry (MS) ? MS grade — low non-volatile and ionic residues compatible with mass spectrometry. Use to minimize background and ion suppression in MS workflows. ActiBioPure™ ? ActiBioPure™ — Aladdin's premier line for bioactive and recombinant products. Use when both high purity and preserved biological activity are required. EnzymoPure™ ? EnzymoPure™ — Aladdin's line of high-quality enzymatic solutions. Use when enzyme purity and defined activity drive assay or process performance. for Protein sequencing ? Protein-sequencing grade — ultra-clean reagents for Edman/MS sequencing. Use where contaminants would obscure sequence determination. ≥95%(SDS-PAGE) 100000 U/mL
Accession #
P21163
Expression system
E. coli
Bioactivity
100000 U/mL
 ·  off list, applied to all prices below.
Size
Status
Price
Qty
15KU
rp227283-15KU
1-2 wks(?)
Item is derived from our semi-finished stock and is processed in 1-2 weeks.
$179.90
Enter a quantity for the sizes you want to add.
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Why this grade

Animal Free, Carrier Free, Bioactive, Recombinant, suitable for mass spectrometry (MS), ActiBioPure™, EnzymoPure™, for protein sequencing, ≥95%(SDS-PAGE), 100000 U/mL ActiBioPure™,Animal Free,Bioactive,Carrier Free,for Protein sequencing,Recombinant,Suitable for mass spectrometry (MS),EnzymoPure™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

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Storage & shipping

Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.

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Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

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Literature proof

Cited in 1 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Product Name
PNGase F (MS), CAS No.83534-39-8
Synonyms
N-Glycosidase F | Peptide N-glycosidase | peptide-N(4)-(N-acetyl-beta-glucosaminyl)asparagine amidase | glycopeptidase | glycopeptide N-glycosidase | N-glycanase | N-oligosaccharide glycopeptidase | ngl | PeptideN-Glycosidase F
Grade
ActiBioPure™, Animal Free, Bioactive, Carrier Free, for Protein sequencing, Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™
Specifications & Purity
Animal Free, Carrier Free, Bioactive, Recombinant, suitable for mass spectrometry (MS), ActiBioPure™, EnzymoPure™, for protein sequencing, ≥95%(SDS-PAGE), 100000 U/mL
Biochemical and Physiological Mechanisms
Cleaves an entire glycan from a glycoprotein. Requires that the glycosylated asparagine moiety (reaction 1) be substituted on its amino (R1) and carboxyl (R2) terminus with a polypeptide chain. PNGase F binds primarily to the polypeptide chain around the
Bioactivity
100000 U/mL
Accession #
Predicted molecular weight
36 kDa
CAS
83534-39-8
Enzyme Commission Number
3.5.1.52
Molecule Type
Enzyme
Storage and Shipping
Concentration
100000 U/mL
Storage
Store at -20°C,Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
Stability And Storage
Store at -20℃ long term (1 year). Avoid freeze/thaw cycle.
Unit definition
The amount of enzyme required to remove >95% of carbohydrates from 10^4 g of denatured RNase B in 1 hour at 37°C, with a total reaction volume of 10 μl.

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

2 results found

Lot NumberCertificate TypeDateItem
ZJ26F0636797Certificate of AnalysisJun 29, 2026 rp227283
ZJ25F0926879Certificate of AnalysisJun 14, 2026 rp227283
Documents & Articles
Citations of This Product
References
1. Donghua Su, Xiaolong Zhang, Tianwu An, Xi Wang, Yaqiu Lin, Haibo Wang, Peiqiang Yu, Haitao Shi.  (2025)  Astral-DIA proteomics and 4D label-free N-glycoproteomics of milk fat globule membrane proteins reveal distinct profiles between yak colostrum and mature milk.  Food Chemistry-X,      [PMID:41378274] [10.1016/j.fochx.2025.103302]
Solution Calculators
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