Protease Subtilisin A, CAS No.9014-01-1

CAS: 9014-01-1 Cat. No.: P755356 EC Number: 232-752-2

GRADE & PURITY Native, EnzymoPure™, from Bacillus licheniformis;>8 U/mg protein

Accession #

P00780

Bioactivity

>8 U/mg protein

Overview

High purity Protease (Subtilisin A from Bacillus licheniformis) (Powder) for use in research, biochemical enzyme assays and in vitro diagnostic analysis.

PROPERTIES

1. ELECTROPHORETIC PURITY:

- Single major band on SDS-PAGE (MW = 30,250); minor bands at MW < 30,000

- Single major band on isoelectric focusing (pI 8.8); minor band at pI 9.3

2. PHYSICOCHEMICAL PROPERTIES:

pH Optima: 7.0-7.5 (60% of maximal activity at pH 6.0)

pH Stability: 5.5-10.0

Temperature Optima: 60°C

Temperature Stability: < 60°C

This enzyme is suitable for use in AOAC INTERNATIONAL Total Dietary Fiber analytical procedures and is recommended for AOAC 2001.03.

Specifications

Product Name

Protease Subtilisin A, CAS No.9014-01-1

Synonyms

Proteinase from Bacillus licheniformis | Subtilisin A | Subtilisin Carslberg | subtilisin | subC

Grade

Native, EnzymoPure™

Specifications & Purity

EnzymoPure™, Nature, from Bacillus licheniformis;>8 U/mg protein

Biochemical and Physiological Mechanisms

Subtilisin is an extracellular alkaline serine protease, it catalyzes the hydrolysis of proteins and peptide amides. Shows high specificity for aromatic and hydrophobic amino acids in the P1 substrate position. May play an important role in the degradatio

Bioactivity

>8 U/mg protein

Accession #

P00780

CAS

9014-01-1

Enzyme Commission Number

3.4.21.62

Molecule Type

Enzyme

Product Line

Biological Products

Storage and Shipping

Concentration

from Bacillus licheniformis;>8 U/mg protein

Reconstitution

Accurately weigh 2.5g of protease powder and prepare a solution containing 50 mg of protease per milliliter using 50 mL of 50 mmol/L maleic acid buffer. Vortex and shake for 5 minutes before use. Store at 4 ℃ before use.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (＞4 years).

Unit definition

One Unit will hydrolyse casein to produce colour equivalent to one μmole (181 μg) of tyrosine per minute at pH 7.0 at 40°C (colour by Folin-Ciocalteu reagent).