Recombinant Carboxypeptidase B, CAS No.9025-24-5

CAS: 9025-24-5 Cat. No.: S141076 Molecular Weight: 610.7 EC Number: 232-788-9 PubChem CID: 3289209

GRADE & PURITY Animal Free, Carrier Free, Bioactive, Recombinant, Suitable for mass spectrometry (MS), ActiBioPure™, EnzymoPure™, ≥95%(HPLC), ≥200 U/mg protein, expressed in E.coli

Accession #

P19223

Expression system

E. coli

Bioactivity

≥200 U/mg protein

Overview

Carboxypeptidase B (Carboxypeptidase B), also known as peptidyl - L - lysine (L - arginine) hydrolase and arginase, is a metalloprotease that can specifically hydrolyze basic amino acids (lysine, arginine, histidine) at the C - terminus of proteins.

Enzymatic cleavage site: Carboxypeptidase B specifically hydrolyzes the amino terminus of basic amino acids (lysine (Lysine, K), arginine (Arginine, R), histidine (Histidine, H)) at the C - terminus of proteins.
Amino acid sequence: Yaxin recombinant carboxypeptidase B is produced by recombinant Escherichia coli expression, and its amino acid sequence is completely consistent with that of rat pancreatic carboxypeptidase B.
Enzymological properties: It has the same enzymological properties as animal - derived carboxypeptidase B and can replace animal - derived carboxypeptidase B in various biotechnology processes.
Basic characteristics: Also known as peptidyl - L - lysine (L - arginine) hydrolase; arginase. The molecular weight is 33.8 kD, the isoelectric point is 6.0, and the optimal pH is 7.5 - 9.0. Its activity is competitively inhibited by arginine and lysine, and metal ion chelators such as EDTA inhibit the enzyme activity.

Usage Method
Dissolution: Dissolve to a final concentration of 1 - 10 mg/ml in pure water or 25 mM Tris - HCl (pH 7.5 - 8.0).
Usage: Enzyme amount: target protein = 1:20 - 1:100 (w:w), and the optimal enzymatic cleavage pH is 7.0 - 9.0.

Storage Stability
The lyophilized powder of recombinant carboxypeptidase B is stable for 24 months when stored at 2 - 8°C; after being dissolved in sterile water or 25 mM Tris - HCl (pH 7.6) and stored at - 20°C, there is no loss of activity after repeated freezing and thawing for 10 times.

Product Advantages
Sequence - analyzed pure protease: high specificity and good stability.
Animal - free origin: produced by recombination, free from exogenous virus contamination, and no animal - derived raw materials are used in the production process.
Stable quality: mass production can ensure stable and continuous batch production; there is no difference between product batches, and the quality is stable.
High purity: high specific activity; residual host protein is lower than the limit requirements for biological products.
Lyophilized powder: easy to store and transport.
Compliance with regulatory requirements: production equipment and production environment meet relevant regulatory requirements, and the production process fully follows the NSF ISO 9001:2015 quality system and conforms to GMP guidelines.
Complete quality documents: relevant regulatory support documents can be provided according to customer needs.

Product Applications
Detection of antibody basic peaks;
Determination of amino acids at the C - terminus of proteins.

Specifications

Product Name

Recombinant Carboxypeptidase B, CAS No.9025-24-5

Synonyms

DTXSID801009277 | FT-0623486 | Carboxypeptidase B from human pancreas, 50-55 units/mg protein carboxypeptidase B | 4-[[1-[[1-[2-[(1-benzylsulfanyl-1-oxo-3-phenylpropan-2-yl)carbamoyl]pyrrolidin-1-yl]-1-oxopropan-2-yl]amino]-1-oxopropan-2-yl]amino]-4-oxobu

Grade

ActiBioPure™, Animal Free, Bioactive, Carrier Free, Recombinant, Suitable for mass spectrometry (MS), EnzymoPure™

Specifications & Purity

EnzymoPure™, ActiBioPure™, Bioactive, Animal Free, Carrier Free, suitable for mass spectrometry (MS), Recombinant, ≥95%(HPLC), ≥200 U/mg protein, expressed in E.coli

Biochemical and Physiological Mechanisms

Preferential release of a C-terminal lysine or arginine amino acid.

Bioactivity

≥200 U/mg protein

Accession #

P19223

Predicted molecular weight

33.6 kDa

CAS

9025-24-5

Enzyme Commission Number

EC: 3.4.17.2

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥200 U/mg protein, expressed in E.coli

Reconstitution

Reconstitute in pure water or 25mM Tris-HCl (pH 7.5-8.0) to a concentration of 1-10 mg/ml.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Unit definition

The amount of enzyme that catalyzes the hydrolysis of 1 μmol of hippuric acid - L - arginine in 1 minute under the conditions of 25°C and pH 7.65 is 1 U.

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Amino acids, peptides, and analogues
        • Level5 Peptides
          • Level6 Oligopeptides

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Amino acids, peptides, and analogues
• Intermediate Tree Nodes: Peptides
• Direct Parent: Oligopeptides
• Alternative Parents: Phenylalanine and derivatives N-acyl-alpha amino acids and derivatives Proline and derivatives Alpha amino acid amides Alanine and derivatives Amphetamines and derivatives N-acylpyrrolidines Pyrrolidinecarboxamides N-acyl amines Tertiary carboxylic acid amides Thioesters Secondary carboxylic acid amides Carbothioic S-esters Monocarboxylic acids and derivatives Carboxylic acids Azacyclic compounds Sulfenyl compounds Organonitrogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds Organopnictogen compounds
• Molecular Framework: Aromatic heteromonocyclic compounds
• Substituents: Alpha-oligopeptide - Phenylalanine or derivatives - Proline or derivatives - N-acyl-alpha amino acid or derivatives - Alpha-amino acid amide - Alanine or derivatives - N-substituted-alpha-amino acid - Alpha-amino acid or derivatives - Amphetamine or derivatives - N-acylpyrrolidine - Pyrrolidine carboxylic acid or derivatives - Pyrrolidine-2-carboxamide - Monocyclic benzene moiety - Fatty amide - N-acyl-amine - Fatty acyl - Benzenoid - Tertiary carboxylic acid amide - Pyrrolidine - Secondary carboxylic acid amide - Carbothioic s-ester - Thiocarboxylic acid ester - Carboxamide group - Organoheterocyclic compound - Sulfenyl compound - Thiocarboxylic acid or derivatives - Azacycle - Carboxylic acid - Monocarboxylic acid or derivatives - Organic oxide - Organic oxygen compound - Organopnictogen compound - Hydrocarbon derivative - Carbonyl group - Organic nitrogen compound - Organonitrogen compound - Organooxygen compound - Organosulfur compound - Aromatic heteromonocyclic compound
• Description: This compound belongs to the class of organic compounds known as oligopeptides. These are organic compounds containing a sequence of between three and ten alpha-amino acids joined by peptide bonds.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y