Toluidine bule - Biological stain , CAS No.6586-04-5

CAS: 6586-04-5 Cat. No.: T104232 Formula: C₁₅H₁₆ClN₃S·0.5ZnCl₂ Molecular Weight: 373.97 Beilstein Registry Number: 4068052 PubChem CID: 11456928

GRADE & PURITY Biological Stain

Synonyms

(7-amino-8-methylphenothiazin-3-ylidene)-dimethylazanium;dichlorozinc;dichloride | DTXSID00466405 | SBB058282 | Toluidine Blue, for microscopy (Hist., Vit.) | AKOS024375442

Storage

Desiccated,Room temperature

Shipped In

Normal

Overview

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals. It is found to have affinity for nucleic acids, and therefore binds to nuclear material of tissues with a high DNA and RNA content

Application：

Toluidine Blue is suitable for use in the following applications:
to demonstrate amyloid; DNA in plant material, mast cells, Nissl substance, nuclei, and sulfatides
in metachromatic staining of sulfated mucins and related materials
as staining agent to identify the development and accumulation of the cartilage matrix, proteoglycans for isolation and culture of mesenchymal stem cells from mouse compact bone.
staining of mucins in water-miscible resins and of nucleic acids in epoxy resins(1)

Specifications

Synonyms

(7-amino-8-methylphenothiazin-3-ylidene)-dimethylazanium;dichlorozinc;dichloride | DTXSID00466405 | SBB058282 | Toluidine Blue, for microscopy (Hist., Vit.) | AKOS024375442

Specifications & Purity

Biological stain

Biochemical and Physiological Mechanisms

Toluidine Blue is a basic thiazine metachromatic dye, which is used for selectively staining acidic tissue components such as carboxylates, sulfates, and phosphate radicals.It is found to have an affinity for nucleic acids and therefore binds to the nucle

Storage

Desiccated,Room temperature

Shipped In

Normal

Grade

Biological Stain

Names and Identifiers

Pubchem Sid

488197521

Pubchem Sid Url

https://pubchem.ncbi.nlm.nih.gov/substance/488197521

Canonical Smiles

CC1=CC2=C(C=C1N)SC3=CC(=[N+](C)C)C=CC3=N2.CC1=CC2=C(C=C1N)SC3=CC(=[N+](C)C)C=CC3=N2.[Cl-].[Cl-].Cl[Zn]Cl

IUPAC Name

(7-amino-8-methylphenothiazin-3-ylidene)-dimethylazanium;dichlorozinc;dichloride

InChIKey

DAQXTSXITPUGRG-UHFFFAOYSA-L

INCHI

1S/2C15H15N3S.4ClH.Zn/c2*1-9-6-13-15(8-11(9)16)19-14-7-10(18(2)3)4-5-12(14)17-13;;;;;/h2*4-8,16H,1-3H3;4*1H;/q;;;;;;+2/p-2

Isomeric SMILES

CC1=CC2=C(C=C1N)SC3=CC(=[N+](C)C)C=CC3=N2.CC1=CC2=C(C=C1N)SC3=CC(=[N+](C)C)C=CC3=N2.[Cl-].[Cl-].Cl[Zn]Cl

WGK Germany

3

PubChem CID

11456928

Molecular Weight

373.97

Beilstein

4068052

Chemical and Physical Properties

• Solubility: Solubility: Soluble in water

Frequently Asked Questions

What does Biological Stain mean?

Biological Stain indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at room temperature, desiccated. Keep the container tightly closed with desiccant — the material is moisture-sensitive.

How is this product shipped?

This product ships under standard ambient conditions. No temperature-controlled packaging is required.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 6586-04-5, the molecular formula is C15H16ClN3S·0.5ZnCl2, and the molecular weight is 373.97 g/mol. InChIKey DAQXTSXITPUGRG-UHFFFAOYSA-L.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.