Transferrin (Partially Iron Saturated) from Human Plasma, CAS No.11096-37-0

CAS: 11096-37-0 Cat. No.: T754984

GRADE & PURITY BioReagent, Native, Tissue Culture Grade, ≥98%(SDS-PAGE), Iron Content: 300-600μg/g; Pre-lyophilization Protein Concentration: See COA

Accession #

P02787

Overview

  Transferrin is a glycoprotein with a branched carbohydrate chain, having two sites for ferric ion binding in its polypeptide chain.

Application

Transferrin human has been used:

• to incubate human proximal tubule cells (HKC) - 8 cells along with IgG for the examination of albumin specificity;
• as a supplement in serum-containing/devoid medium for human thyroid cell culture;
• as a target glycoprotein to develop an in-gel hydrazinolysis method.

Biochem/physiol Actions

Transferrin mediates iron transportation from plasma to cells. The iron-bound transferrin are of three forms: monoferric A/B transferrin and diferric transferrin. The transferrin binds to specific cell surface receptors and are internalized by endocytosis. Once inside, the iron is released into the cells and the iron-free transferrin, also referred to as apotransferrin, is exocytosed outside the cell without being degraded. Transferrin is known to deliver iron to cells in a physiologically safe and effective form.

Preparation Note

Heat treated at 60°C for 10 hours.

Other Notes

Partially iron-saturated

Iron Content: 300-600 ug Fe/g protein

Prepared from plasma shown to be non reactive for HBsAg, anti-HCV, anti-HBc, and negative for anti-HIV 1 & 2 by FDA-required tests.

Specifications

Product Name

Transferrin (Partially Iron Saturated) from Human Plasma, CAS No.11096-37-0

Synonyms

Siderophilin, partially saturated | Serotransferrin | TF | TFQTL1 | PRO1557 | PRO2086 | HEL-S-71p

Grade

BioReagent, Native, Tissue Culture Grade

Specifications & Purity

BioReagent, Native, Tissue Culture Grade, ≥98%(SDS-PAGE), Iron Content: 300-600μg/g; Pre-lyophilization Protein Concentration: See COA

Biochemical and Physiological Mechanisms

Transferrins are iron binding transport proteins which can bind two Fe3+ ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage a

Accession #

P02787

Predicted molecular weight

80 kDa

CAS

11096-37-0

Molecule Type

Protein

Product Line

Biological Products

Product Properties

ALogP

-2.5

Storage and Shipping

Concentration

Iron Content: 300-600μg/g; Pre-lyophilization Protein Concentration: See COA

Reconstitution

After centrifugation, we suggest addition of de-ionized H2O to the original volume (See COA), followed by gentle mixingto ensure adequate homogenization. If further dilution is required, please use the lyophilization buffer.

Storage

Store at -20°C,Avoid repeated freezing and thawing

Shipped In

Ice chest + Ice pads

Stability And Storage

Store at -20℃ long term (>1 year). Upon reconstitution, it is recommended to aliquot. Avoid freeze/thaw cycle.

Images

SDS-PAGE: Transferrin (Partially Iron Saturated) from Human Plasma (T754984)

Transferrin (Partially Iron Saturated) from Human Plasma (T754984) - SDS-PAGE 

2 μg/lane of Transferrin (Partially Iron Saturated) from Human Plasma was resolved with SDS-PAGE under reducing (R) and non-reducing (N) conditions and visualized by Coomassie® Blue staining, showing the band at 76 kDa under reducing condition and 65 kDa under non-reducing condition.

Frequently Asked Questions

What is the purity of this product, and how is it determined?

This product is supplied at ≥98% purity, determined by SDS-PAGE. Lot-specific values are stated on the Certificate of Analysis.

What does Native mean?

Native indicates that the material has been processed and tested for specialty industrial processing. Specifications focus on the impurities that affect performance in that application — for example, metal content for electronics, particle size for batteries, refractive index for optics, or moisture content for moisture-sensitive processes.

What does BioReagent mean?

BioReagent indicates that the product has been processed and tested for biological and biochemical applications. Specifications may include endotoxin limits, microbial controls, sterility, low DNase/RNase/protease activity, and verified biological activity where relevant.

How should this product be stored?

Store at ?20 °C. Avoid repeated freeze–thaw cycles. Aliquot into single-use volumes before freezing.

How is this product shipped?

This product ships in an insulated container with ice pads. Unpack on arrival and transfer it to the storage condition stated above.

What are the CAS number, molecular formula and molecular weight?

The CAS Number is 11096-37-0.

What documentation is provided?

Available product documentation, including Certificates of Analysis (COA), Safety Data Sheets (SDS), and specification sheets, is shown in the product document area. Document availability and access follow the current site policy.