Tunicamycin - ≥98% , CAS No.11089-65-9

CAS: 11089-65-9 Cat. No.: T101151 Molecular Weight: 844.95 Beilstein Registry Number: 6888090 EC Number: 601-012-4

GRADE & PURITY ≥98%

Synonyms

Tunicamycin fromStreptomycessp.

Storage

Protected from light,Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Tunicamycin is a mixture of Tunicamycins A, B, C and D which has been widely used in the study of glycoprotein synthesis in various biological systems. Tunicamycin inhibits GNPTAB (GlcNAc phosphotransferase, GPT) and inhibts the formation of N-glycosidic linkages in glycoprotein synthesis. Tunicamycin has also been reported to have a dose-dependent inhibition of DNA synthesis, to inhibit protein glycosylation, to suppress the S-phase of the cell cycle, and to arrest the cell cycle in late G1. As a member of a family of antibiotics produced by Streptomyces lysosuperficus, this compound is noted to be active in vitro against gram-positive bacteria, fungi, yeasts and viruses. During protein glycosylation, tunicamycin is noted to be an inhibitor of the transfer of saccharide moieties to dolichol during dolichol-linked glycoprotein synthesis. Dose-dependent inhibition of DNA synthesis may be related to the alteration of glycoproteins, which thereby affects the transport of thymidine into cells. Additionally, tunicamycin has been reported to prevent cell cycle progression in primary cultures of rat glial cells, as well as inhibit lipid-mediated protein glycosylation in chick or mouse fibroblasts in a dose-dependent manner.
A competitive cell cycle inhibitor

Specifications

Synonyms

Tunicamycin fromStreptomycessp.

Specifications & Purity

≥98%

Biochemical and Physiological Mechanisms

Antibacterial and antifungal. Blocks the formation of protein N-glycosidic linkages by inhibiting the transfer of N-acetylglucosamine 1-phosphate to dolichol monophosphate. Inhibits bacterial and eukaryote N-acetylglucosamine transferases and prevents for

Storage

Protected from light, Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Toxic, refer to SDS for further information. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥98%

Names and Identifiers

• Pubchem Sid: 504773823
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504773823
• WGK Germany: 3
• RTECS: YO7980200
• UN Number: 3462
• Molecular Weight: 844.95
• Beilstein: 6888090

Chemical and Physical Properties

• Solubility: Soluble in water (partly miscible), alkaline water, pyridine, hot methanol, DMSO (>10 mg/ml), ethanol (5 mg/ml, warm), butanol (slightly), and DMF. Insoluble in acetone, ethyl acetate, chloroform, benzene, and acidic water.
• Sensitivity: Moisture sensitive;Light sensitive

References

1. Fengying Dai, Kepeng Lv, Bo Zhang, Junqiang Zhao, Shaoteng Wang, Ke Lan, Yiping Zhao, Xiaolei Zhang, Bohong Kan.  (2023)  Overcoming the structure deficiency of nanodrug coated with tannic acid shell through phenolic hydroxyl protection strategy for Alzheimer's disease combination treatment.  Biomaterials Advances,      [PMID:37827021] [10.1016/j.bioadv.2023.213651]

2. Shuanghui Wang, Chuhong Chen, Yan Lu.  (2023)  Epigallocatechin-3-Gallate Reduces Cd-Induced Developmental Toxicity of Bodysize in Caenorhabditis elegans via the PEK-1/eIF-2α/ATF-4 Pathway.  MOLECULES,  28  (17): (6344).  [PMID:37687170] [10.3390/molecules28176344]

3. Chuhong Chen, Ying Deng, Linyan Liu, Zhenyan Zou, Chenzhong Jin, Zhiyin Chen, Shuanghui Wang.  (2023)  High-Dose Deltamethrin Induces Developmental Toxicity in Caenorhabditis elegans via IRE-1.  MOLECULES,  28  (17): (6303).  [PMID:37687132] [10.3390/molecules28176303]

4. Ling-Yan Wang, Jian Li, Benqiang Gong, Rui-Hua Wang, Yi-Li Chen, Jian Yin, Chang Yang, Jia-Ting Lin, Hao-Zhuo Liu, Yubing Yang, Jianfeng Li, Chunyu Li, Nan Yao.  (2023)  Orosomucoid proteins limit endoplasmic reticulum stress in plants.  NEW PHYTOLOGIST,      [PMID:37606093] [10.1111/nph.19200]

5. Gao Xiu Kui, Sheng Zu Kang, Lu Ye Hong, Sun Yu Ting, Rao Xi Sheng, Shi Lin Jing, Cong Xiao Xia, Chen Xiao, Wu Hao Bo, Huang Man, Zheng Qiang, Guo Jian-sheng, Jiang Liang Jun, Zheng Li Ling, Zhou Yi Ting.  (2023)  VAPB-mediated ER-targeting stabilizes IRS-1 signalosomes to regulate insulin/IGF signaling.  Cell Discovery,  9  (1): (1-18).  [PMID:37528084] [10.1038/s41421-023-00576-6]

6. Ting Xiao, Yuan Zhi, Fangfang Tian, Feilong Huang, Xingyan Cheng, Ai Wu, Ling Tao, Zhenghong Guo, Xiangchun Shen.  (2023)  Ameliorative effect of black raspberry anthocyanins on diabetes retinopathy by inhibiting axis protein tyrosine phosphatase 1B-endoplasmic reticulum stress.  Journal of Functional Foods,      [PMID:] [10.1016/j.jff.2023.105696]

7. Xue Zhichao, Zeng Jiaming, Yin Xinchi, Li Yongshu, Meng Bo, Zhao Yang, Fang Xiang, Gong Xiaoyun, Dai Xinhua.  (2023)  Investigation on acquired palbociclib resistance by LC-MS based multi-omics analysis.  Frontiers in Molecular Biosciences,      [PMID:36743215] [10.3389/fmolb.2023.1116398]

8. Nianxiu Tian, Yanbin Gao, Xiaolei Wang, Xiaoming Wu, Dawei Zou, Zhiyao Zhu, ZheJi Han, Tao Wang, Yimin Shi.  (2022)  Emodin mitigates podocytes apoptosis induced by endoplasmic reticulum stress through the inhibition of the PERK pathway in diabetic nephropathy.  Drug Design Development and Therapy,      [PMID:30034224] [10.2147/DDDT.S167405]

9. Jiang Kai-long, Liu Chang-mei, Nie Li-tong, Jiang Hai-ni, Xu Lei, Zhang Kun-zhi, Fan Li-xia, Gao An-hui, Lin Lu-ling, Wang Xiang-yu, Tan Ming-jia, Zhang Qi-qing, Zhou Yu-bo, Li Jia.  (2022)  Discovery of toxoflavin, a potent IRE1α inhibitor acting through structure-dependent oxidative inhibition.  ACTA PHARMACOLOGICA SINICA,  44  (1): (234-243).  [PMID:35840659] [10.1038/s41401-022-00949-9]

10. Xijie Yu, Xiaoxiao Xu, Wei Dong, Chang Yang, Yao Luo, Ying He, Chenxi Jiang, Yanru Wu, Jiawei Wang.  (2022)  DDIT3/CHOP mediates the inhibitory effect of ER stress on chondrocyte differentiation by AMPKα-SIRT1 pathway.  BIOCHIMICA ET BIOPHYSICA ACTA-MOLECULAR CELL RESEARCH,      [PMID:35381294] [10.1016/j.bbamcr.2022.119265]

11. Wang Zijing, Ma Peng, Wang Yisa, Hou Biyu, Zhou Can, Tian He, Li Bowen, Shui Guanghou, Yang Xiuying, Qiang Guifen, Yin Chengqian, Du Guanhua.  (2021)  Untargeted metabolomics and transcriptomics identified glutathione metabolism disturbance and PCS and TMAO as potential biomarkers for ER stress in lung.  Scientific Reports,  11  (1): (1-13).  [PMID:34282162] [10.1038/s41598-021-92779-8]

12. Peng Ma, Zijing Wang, Yisa Wang, Biyu Hou, Jialin Sun, He Tian, Bowen Li, Guanghou Shui, Xiuying Yang, Xinyu Yang, Guifen Qiang, Chong Wee Liew, Guanhua Du.  (2021)  Integration of Metabolomics and Transcriptomics Reveals Ketone Body and Lipid Metabolism Disturbance Related to ER Stress in the Liver.  JOURNAL OF PROTEOME RESEARCH,      [PMID:34270263] [10.1021/acs.jproteome.1c00167]

13. Wenli Shi, Danyi Lu, Chunlei Wu, Meiqing Li, Zhihao Ding, Yanyan Li, Binghua Chen, Xian Lin, Wu Su, Ximing Shao, Zhihui Xia, Lijing Fang, Ke Liu, Hongchang Li.  (2021)  Coibamide A kills cancer cells through inhibiting autophagy.  BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS,      [PMID:33592379] [10.1016/j.bbrc.2021.01.112]

14. Tian Jia-hui, Wu Qian, He Yong-xiang, Shen Qi-ying, Rekep Mubarak, Zhang Gui-ping, Luo Jian-dong, Xue Qin, Liu Ying-hua.  (2020)  Zonisamide, an antiepileptic drug, alleviates diabetic cardiomyopathy by inhibiting endoplasmic reticulum stress.  ACTA PHARMACOLOGICA SINICA,  42  (3): (393-403).  [PMID:32647341] [10.1038/s41401-020-0461-z]

15. Yanan Chen, Hao Zhang, Yueping Chen, Yuying Zhang, Mingming Shen, Peilu Jia, Shuli Ji, Tian Wang.  (2020)  Resveratrol Alleviates Endoplasmic Reticulum Stress–Associated Hepatic Steatosis and Injury in Mice Challenged with Tunicamycin.  MOLECULAR NUTRITION & FOOD RESEARCH,  64  (14): (2000105).  [PMID:32529694] [10.1002/mnfr.202000105]

16. Damu Lin, Hongqiang Wu, Zongwei Zhou, Zhenyu Tao, Tanghong Jia, Weiyang Gao.  (2019)  Ginkgolide B improves multiterritory perforator flap survival by inhibiting endoplasmic reticulum stress and oxidative stress.  JOURNAL OF INVESTIGATIVE SURGERY,      [PMID:31870195] [10.1080/08941939.2019.1676483]

17. Zewei Luo, Yimin Wang, Ya Xu, Xu Wang, Zhijun Huang, Junman Chen, Yongxin Li, Yixiang Duan.  (2019)  Ultrasensitive U-shaped fiber optic LSPR cytosensing for label-free and in situ evaluation of cell surface N-glycan expression.  SENSORS AND ACTUATORS B-CHEMICAL,      [PMID:] [10.1016/j.snb.2019.01.015]

18. Xiaoyu Wang, Xuejie Han, Minghui Li, Yu Han, Yun Zhang, Shiqi Zhao, Yue Li.  (2018)  Ticagrelor protects against AngII-induced endothelial dysfunction by alleviating endoplasmic reticulum stress.  MICROVASCULAR RESEARCH,      [PMID:29777791] [10.1016/j.mvr.2018.05.006]

19. Yimin Wang, Zhao Zhao, Fujing Wei, Zewei Luo, Yixiang Duan.  (2018)  Combining autophagy-inducing peptides and brefeldin A delivered by perinuclear-localized mesoporous silica nanoparticles: a manipulation strategy for ER-phagy.  Nanoscale,  10  (18): (8796-8805).  [PMID:29713715] [10.1039/C8NR00872H]

20. Zihan Wang, Zhuowei Lei, Quanji Wang, Qian Jiang, Zhuo Zhang, Xiaojin Liu, Biao Xing, Sihan Li, Xiang Guo, Yanchao Liu, Xingbo Li, Yiwei Qi, Kai Shu, Huaqiu Zhang, Yimin Huang, Ting Lei.  (2024)  Connexin 36 Mediated Intercellular Endoplasmic Reticulum Stress Transmission Induces SSTA Resistance in Growth Hormone Pituitary Adenoma.  International Journal of Biological Sciences,      [PMID:38169563] [10.7150/ijbs.86736]

21. Ma Lin-Wei, Liu Yu-Fan, Zhang Hui, Huang Chang-Jun, Li Ang, Qu Xin-Zhe, Lin Jia-Piao, Yang Yan, Yao Yong-Xing.  (2024)  Electroacupuncture attenuates neuropathic pain via suppressing BIP-IRE-1α-mediated endoplasmic reticulum stress in the anterior cingulate cortex.  BIOLOGICAL RESEARCH,  57  (1): (1-12).  [PMID:38812057] [10.1186/s40659-024-00511-3]

22. Cao Thang Luong, Gilbert Audira, Kevin Adi Kurnia, Chih-Hsin Hung, Chung-Der Hsiao.  (2024)  Fish 3D Locomotion app: a user-friendly computer application package for automatic data calculation and endpoint extraction for novel tank behavior in fish.  JOURNAL OF FISH BIOLOGY,  105  (4): (1086-1108).  [PMID:39007187] [10.1111/jfb.15860]

23. Jie Jiang, Yuqing Gao, Jiang Wang, Yan Huang, Rong Yang, Yongxin Zhang, Yuandi Ma, Yingquan Wen, Gongkai Luo, Shurui Zhang, Yutang Cao, Minjun Yu, Qinxue Wang, Shulei Hu, Kanglong Wang, Xiaozhen Guo, Frank J. Gonzalez, Yameng Liu, Hong Liu, Qing Xie, Cen Xie.  (2025)  Hepatic sphingomyelin phosphodiesterase 3 promotes steatohepatitis by disrupting membrane sphingolipid metabolism.  Cell Metabolism,      [PMID:40015281] [10.1016/j.cmet.2025.01.016]

24. Yuan Gao, Jiao Xu, Kaichuan He, Qi Guo, Lin Xiao, Sheng Jin, Danyang Tian, Xu Teng, Cuixia An, Hongmei Xue, Yuming Wu.  (2024)  Hydrogen Sulfide Ameliorated Endothelial Dysfunction in hyperhomocysteinemia Rats: Mechanism of IRE1α/JNK pathway-mediated Autophagy.  NITRIC OXIDE-BIOLOGY AND CHEMISTRY,      [PMID:39396802] [10.1016/j.niox.2024.10.008]

25. Tan Yue-Rong, Shen Si-Yang, Li Xin-Yi, Yi Peng-Fei, Fu Ben-Dong, Peng Lu-Yuan.  (2024)  Mogroside V reduced the excessive endoplasmic reticulum stress and mitigated the Ulcerative colitis induced by dextran sulfate sodium in mice.  Journal of Translational Medicine,  22  (1): (1-12).  [PMID:38773576] [10.1186/s12967-024-05285-6]

26. Cao Ting, Wang Xue-lian, Rao Jiang-yan, Zhu Hui-feng, Qi Hong-yi, Tian Zhen.  (2024)  Periplaneta americana L. extract exerts neuroprotective effects by inhibiting endoplasmic reticulum stress via AKT-dependent pathway in experimental models of Parkinson’s disease.  Chinese Medicine,  19  (1): (1-24).  [PMID:39538357] [10.1186/s13020-024-01029-2]

27. Xing Li, Bingtian Xu, Lu Long, Yuting Li, Xuan Xiao, Shuqin Qiu, Jiangping Xu, Li-Wen Tian, Haitao Wang.  (2024)  Phelligridimer A enhances the expression of mitofusin 2 and protects against cerebral ischemia/reperfusion injury.  CHEMICO-BIOLOGICAL INTERACTIONS,      [PMID:38825057] [10.1016/j.cbi.2024.111090]

28. Wang Xiaolu, Wang Qianqian, Wang Hong, Cai Guodi, An Yana, Liu Peiqing, Zhou Huihao, Chen Hong-Wu, Ji Shufeng, Ye Jiantao, Wang Junjian.  (2025)  Small protein ERSP encoded by LINC02870 promotes triple negative breast cancer progression via IRE1α/XBP1s activation.  CELL DEATH AND DIFFERENTIATION,      [PMID:39799200] [10.1038/s41418-025-01443-5]

29. Yanran Huang, Zhaofeng Xu, Yan Yan, Chunbo Li, Jieying Yan, Yi Wei, Jian Li, Luo Zhang, Nan Zhang, Claus Bachert, Weiping Wen.  (2025)  Excessive Endoplasmic Reticulum Stress in B Cells Associates With the Local Immunoglobulin Production in Severe Type 2 Chronic Rhinosinusitis With Nasal Polyps.  CLINICAL AND EXPERIMENTAL ALLERGY,      [PMID:40613328] [10.1111/cea.70107]

30. Wei Liangli, Yuan Yuqi, Yang Ziteng, Li Yuqing, Wang Tingting, Hu Shenglin, Cai Biao, Wang Guangyun.  (2024)  Ginsenoside Rb1 reduced ischemic stroke-induced apoptosis through endoplasmic reticulum stress-associated IRE1/TRAF2/JNK pathway.  NAUNYN-SCHMIEDEBERGS ARCHIVES OF PHARMACOLOGY,      [PMID:39052059] [10.1007/s00210-024-03292-4]

31. Jing Ren, Qi Wang, Xiaxia Zhang, Yongheng Cao, JingXia Wu, Juan Tian, Yanjun Yu, Qingqiu Gong, Zhaosheng Kong.  (2025)  Control of Rhizobia Endosymbiosis by Coupling ER Expansion with Enhanced UPR.  Advanced Science,      [PMID:39985282] [10.1002/advs.202414519]

32. Ren Tingju, Zhang Yingzhi, Tong Yuxiao, Zhang Qi, Wang Tianhao, Wang Yue, Yang Chunguang, Xu Zhangrun.  (2025)  FRET imaging of glycoRNA on small extracellular vesicles enabling sensitive cancer diagnostics.  Nature Communications,  16  (1): (1-19).  [PMID:40210865] [10.1038/s41467-025-58490-2]

33. Xinyu Zhang, Haojie Liu, Yumeng Shi, Zhenhao Liu, Yan Wang, Simai Chen, Xingzhi Yan, Rongyao Xu, Junqing Ma, Qiang Chen, Shuyu Guo.  (2025)  OPTN ameliorates chondrocyte apoptosis in temporomandibular joint osteoarthritis by modulating ER-mitochondria Ca2+ transfer.  INTERNATIONAL IMMUNOPHARMACOLOGY,      [PMID:40339496] [10.1016/j.intimp.2025.114796]

34. Boya Zhang, Hanze Du, Lei Huang, Lijun Liu, Jing Cui, Linlin Wen, Junguang Liu, Wenhao Zhai, Rongrong Ye, Yuhuan Li, Kexuan Liu, Wangni Xie, Xue Zhang, Xinchen Liu, Huan Wang, Ying Xue, Shi Chen, Hui Pan, Daowei Li, Hongchen Sun.  (2025)  DHA sustains frozen-cell-induced efferocytosis by mitigating endoplasmic reticulum stress to promote inflammation resolution and bone repair.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.169708]

35. Zhibao Zhang, Mingyi Tan, Xin Liu, Wenhua Wang, Xiang Chen, Cong Peng, Shuang Zhao, Lisha Wu.  (2026)  Activation of Unfolded Protein Response Pathways Promotes Keratinocyte Differentiation and Ameliorates Psoriasis Phenotypes.  CELL STRESS & CHAPERONES,      [PMID:41765076] [10.1016/j.cstres.2026.100163]