1,2-Bis(2-aminophenoxy)ethane-N,N,N',N'-tetraacetic Acid - ≥97% , CAS No.85233-19-8

CAS: 85233-19-8 Cat. No.: B124573 Molecular Weight: 476.44 Beilstein Registry Number: 5192406 EC Number: 629-173-6

GRADE & PURITY ≥97%

Synonyms

2,2',2'',2'''-(((Ethane-1,2-diylbis(oxy))bis(2,1-phenylene))bis(azanetriyl))tetraacetic acid | Ca(II)-bapta | CHEBI:60888 | NCGC00166094-01 | 1,2-Bis(2-Aminophenoxy)ethane-N,N,N',N'-tetraacetic acid, 98% | BAPTA, Free Acid | J-002799 | AS-73034 | 1,2-Bis(

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

In nature 1,2-Bis(2-Aminophenoxy)ethane-N,N,N′,N′-tetra acetic acid (BAPTA) is a main metabolic product of BAPTA -AM (BAPTA-tetra(actoxymethyl ester), a neuro protective agent in cerebral ischemia, in rats.[3]BAPTA is an intercellular Ca2+ chelator.
Used to study morphological and signaling events of Ca2+ deficiency.

Specifications

Synonyms

2, 2', 2'', 2'''-(((Ethane-1, 2-diylbis(oxy))bis(2, 1-phenylene))bis(azanetriyl))tetraacetic acid | Ca(II)-bapta | CHEBI:60888 | NCGC00166094-01 | 1, 2-Bis(2-Aminophenoxy)ethane-N, N, N', N'-tetraacetic acid, 98% | BAPTA, Free Acid | J-002799 | AS-73034 | 1, 2-Bis(

Specifications & Purity

≥97%

Biochemical and Physiological Mechanisms

Selective, membrane impermeable extracellular calcium chelator. Binds Ca 2+ ions in a 2:1 ratio. Shows 105-fold greater affinity for Ca 2+ than Mg 2+ . BAPTA-AM and BAPTA sodium salt analog.

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Action Type

INHIBITOR

Note

Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.

Purity

≥97%

Names and Identifiers

• Pubchem Sid: 504756581
• Pubchem Sid Url: https://pubchem.ncbi.nlm.nih.gov/substance/504756581
• Canonical Smiles: C1=CC=C(C(=C1)N(CC(=O)O)CC(=O)O)OCCOC2=CC=CC=C2N(CC(=O)O)CC(=O)O
• IUPAC Name: 2-[2-[2-[2-[bis(carboxymethyl)amino]phenoxy]ethoxy]-N-(carboxymethyl)anilino]acetic acid
• InChIKey: FTEDXVNDVHYDQW-UHFFFAOYSA-N
• INCHI: 1S/C22H24N2O10/c25-19(26)11-23(12-20(27)28)15-5-1-3-7-17(15)33-9-10-34-18-8-4-2-6-16(18)24(13-21(29)30)14-22(31)32/h1-8H,9-14H2,(H,25,26)(H,27,28)(H,29,30)(H,31,32)
• Isomeric SMILES: C1=CC=C(C(=C1)N(CC(=O)O)CC(=O)O)OCCOC2=CC=CC=C2N(CC(=O)O)CC(=O)O
• WGK Germany: 3
• RTECS: MC0423000
• Molecular Weight: 476.44
• Beilstein: 5192406
• Reaxy-Rn: 5192406
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=5192406&ln=

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Carboxylic acids and derivatives
      • Subclass Tetracarboxylic acids and derivatives

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Carboxylic acids and derivatives
• Subclass: Tetracarboxylic acids and derivatives
• Intermediate Tree Nodes: Not available
• Direct Parent: Tetracarboxylic acids and derivatives
• Alternative Parents: Alpha amino acids Aminophenyl ethers Phenoxy compounds Dialkylarylamines Aniline and substituted anilines Alkyl aryl ethers Amino acids Carboxylic acids Organopnictogen compounds Organic oxides Hydrocarbon derivatives Carbonyl compounds
• Molecular Framework: Aromatic homomonocyclic compounds
• Substituents: Tetracarboxylic acid or derivatives - Alpha-amino acid - Alpha-amino acid or derivatives - Aminophenyl ether - Phenoxy compound - Phenol ether - Tertiary aliphatic/aromatic amine - Aniline or substituted anilines - Dialkylarylamine - Alkyl aryl ether - Monocyclic benzene moiety - Benzenoid - Tertiary amine - Amino acid - Amino acid or derivatives - Ether - Carboxylic acid - Organic oxide - Organooxygen compound - Organonitrogen compound - Carbonyl group - Organic nitrogen compound - Amine - Organopnictogen compound - Hydrocarbon derivative - Organic oxygen compound - Aromatic homomonocyclic compound
• Description: This compound belongs to the class of organic compounds known as tetracarboxylic acids and derivatives. These are carboxylic acids containing exactly four carboxyl groups.
• External Descriptors: polyamino carboxylic acid - tetracarboxylic acid

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Associated Targets(Human)

TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 (345557 Activities)

Discover Target: TDP1

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Associated Targets(non-human)

fba Putative fructose-1,6-bisphosphate aldolase (15559 Activities)

Discover Target: fba

Activity Type	Relation	Activity value	Units	Action Type	Journal	PubMed Id	doi	Assay Aladdin ID

Mechanisms of Action

Chemical and Physical Properties

• Solubility: Soluble in sodium bicarbonate.
• Sensitivity: Heat Sensitive
• Melt Point(°C): 174°C
• Molecular Weight: 476.400 g/mol
• XLogP3: 2.200
• Hydrogen Bond Donor Count: 4
• Hydrogen Bond Acceptor Count: 12
• Rotatable Bond Count: 15
• Exact Mass: 476.143 Da
• Monoisotopic Mass: 476.143 Da
• Topological Polar Surface Area: 174.000 Ų
• Heavy Atom Count: 34
• Formal Charge: 0
• Complexity: 613.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 0
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1

References

1. Xinqiang Liang, Mekhrdod S. Kurboniyon, Yuanhan Zou, Kezong Luo, Shuhong Fang, Pengle Xia, Shufang Ning, Litu Zhang, Chen Wang.  (2023)  GSH-Triggered/Photothermal-Enhanced H2S Signaling Molecule Release for Gas Therapy.  Pharmaceutics,  15  (10): (2443).  [PMID:37896203] [10.3390/pharmaceutics15102443]

2. Zijia Liu, Yunjiang Yu, Yuchen Zheng, Chao Sheng, Ao Li, Wenzhuo Li, Zijie Zhao, Yunlu Ning, Renhang Zhou, Wenzhe Hou, Zhixiang Zhou.  (2025)  Lysosomal dependent transcytosis of polystyrene nanoplastics within macrophages.  FOOD AND CHEMICAL TOXICOLOGY,      [PMID:41038372] [10.1016/j.fct.2025.115771]