≥90% for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Protected from light,Store at -20°C,Desiccated Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
The cell-permeant pH indicator, carboxy SNARF-1, acetoxymethyl ester, acetate (CAS #126208-13-7) has a pKa of ~7.5 after de-esterification, thus is useful for measuring pH changes between pH 7 and pH 8. Carboxy-SNARF-1 exhibits a significant pH-dependent emission shift from yellow-orange to deep red fluorescence under acidic and basic conditions, respectively. This pH dependence allows the ratio of the fluorescence intensities from the dye at two emission wavelengths – typically 580 nm and 640 nm – to be used for quantitative determinations of pH. Store at -20℃ in the dark.
Loading Cells with SNARF AM Acetate Esters
Optimal loading conditions for each cell type and experiment should be determined by the researcher. The literature cites a wide range of loading conditions, from 1 to 20 µM SNARF AM acetate incubated with cells for from 10 to 60 minutes. DMSO stock solutions are typically diluted at least 1:1000 into loading buffer to reduce the exposure of cells to DMSO, and the loading buffer should be se rum-free because serum often contains esterase activity. The non ionic detergent Pluronic F-127 is some times used to promote dispersion of the rather nonpolar SNARF AM acetate esters into buffers.
As initial loading conditions, we recommend incubating cells in 1～10 µM SNARF AM acetate for 30 minutes at the optimum temperature for the specific cell type of interest. After loading, cells should be washed before commencing pH measurements. For loading brain slices, incubation for 60 minutes in artificial cerebrospinal fluid(ACSF) containing 20µM carboxy SNARF-1 AM acetate and 4% (w/v) Pluronic F-127 followed by a further 30 minute incubation in dye-free ACSF is recommended.
| Molecular Weight | 567.55 |
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Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| Sensitivity | Sensitive to light and humidity |
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