The Human Hematopoietic Stem Cell Expansion Cytokine Package contains recombinant Flt3 ligand, stem cell factor (SCF), thrombopoietin (TPO), and interleukin-6 (IL-6), critical growth factors and cytokines use in ex vivo culture of hematopoietic stem cells (HSCs). These factors support the proliferation, survival, and differentiation of HSCs.
The package contains the following four vials:
- Animal-Free Recombinant Mouse FLT-3 Ligand Protein (Cat. No. rp153982)
- Animal-Free Recombinant Mouse SCF Protein (Cat. No. rp154627)
- Animal-Free Recombinant Mouse Thrombopoietin Protein (Cat. No. rp154717)
- Animal-Free Recombinant Mouse IL-6 Protein (Cat. No. rp154238)
Flt3 ligand is a growth factor that regulates proliferation of early hematopoietic cells. By itself it does not stimulate proliferation of early hematopoietic cells, but synergizes with other CSFs and interleukins to induce growth and differentiation.
SCF is a hematopoietic growth factor that exerts its activity by signaling through the c-Kit receptor. SCF and c-Kit are essential for the survival, proliferation, and differentiation of hematopoietic cells committed to the melanocyte and germ cell lineages. Human SCF manifests low activity on murine cells, while murine and rat SCF are fully active on human cells.
TPO is a lineage-specific growth factor produced in the liver, kidney, and skeletal muscle. It stimulates the proliferation and maturation of megakaryocytes and promotes increased circulating levels of platelets in vivo.
IL-6 is a pleiotropic cytokine that stimulates B-cell differentiation and antibody production, synergizes with IL-3 in megakaryocyte development and platelet production, induces expression of hepatic acute-phase proteins, and regulates bone metabolism. IL-6 signals through the IL-6 receptor system that consists of two chains, IL-6R and gp130. Recombinant Human IL-6 is a 20.9 kDa protein containing 184 amino acid residues
Information:
| Name | Species | Expression System | Tag | Purity | Activity | Endotoxin | Appearance | Storage | Reconstitution | | Animal-Free Recombinant Mouse FLT-3 Ligand Protein | Mouse | HEK293 | C-hFc & His | ≥90%(SDS-PAGE) | Immobilized Recombinant Mouse FLT3, His Tag at 3 μg/mL (100 μL/well) can bind Recombinant Mouse Flt-3 Ligand, The EC50 of Mouse Flt-3 Ligand is 36 ng/mL. | <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH 7.4 | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. | | Animal-Free Recombinant Mouse SCF Protein | Mouse | HEK293 | C-His | ≥95%(SDS-PAGE) | Measured in a cell proliferation assay using TF-1 human erythroleukemic cells. The ED50 for this effect is 0.5-1.0 ng/mL, corresponding to a specific activity of 1.0×10^6-2.0×10^6 units/mg. | <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. | | Animal-Free Recombinant Mouse Thrombopoietin Protein | Mouse | HEK293 | N-His | ≥90%(SDS-PAGE) | Measured in a cell proliferation assay using MO7e human megakaryocytic leukemic cells. The ED₅₀ for this effect is typically 0.35 ng/mL. | <1.0 EU/μg | Lyophilized | Lyophilized from sterile 10mM PBS, 5% Trehalose, 5% Mannitol, 0.01% Tween-80, pH7.4. | It is recommended that sterile water be added to the vial to prepare a stock solution of 0.25mg/mL. | | Animal-Free Recombinant Mouse IL-6 Protein | Mouse | HEK293
| C-His | ≥95%(SDS-PAGE) | Measured in a cell proliferation assay using NFS-60 mouse myelogenous leukemia lymphoblast cells. The ED50 for this effect is 0.07-0.28 ng/mL, corresponding to a specific activity of 3.57×10^6 ~ 1.43×10^7 units/mg.
| <0.1 EU/μg | Lyophilized | Lyophilized from 10mM PBS, 5% Trehalose, 5% Mannitol, pH7.4. | Reconstitute in sterile water to a concentration of 0.1-0.5 mg/ml. |
|
Notes:
1. This product is for research use only.
2. For your safety and health, please wear a lab coat and disposable gloves during operation.
