GRADE & PURITYSuitable for molecular biology?Molecular-biology grade — free of nucleases and contaminants that degrade DNA/RNA. Use in cloning, PCR, and nucleic-acid work needing clean reagents.BioReagent?BioReagent grade — tested suitable for life-science and molecular-biology use. Use for cell culture, assays, and biochemical work needing biological compatibility.PCR Reagent?PCR grade — nuclease-free, inhibitor-free reagents for reliable amplification. Use in PCR/qPCR where contaminants would block or bias amplification.Sterile?Sterile grade — processed and verified free of viable microorganisms. Use directly in aseptic procedures and cell culture without further sterilization.for DNA and RNA applications?For nucleic-acid (DNA & RNA) applications — nuclease-controlled across both. Use in workflows handling DNA and RNA together where degradation is a risk.10X
Storage
Store at -20°C,Avoid repeated freezing and thawing
BioReagent, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, 10X BioReagent,for DNA and RNA applications,PCR Reagent,Sterile,Suitable for molecular biology for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
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Storage & shipping
Store at -20°C,Avoid repeated freezing and thawing Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
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Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
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Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
Product Introduction:
Aladdin's Annealing Buffer for DNA Oligos (10X) is a buffer solution that has been verified through multiple experiments for the annealing of DNA oligonucleotides. This annealing buffer is not only suitable for the routine annealing of DNA oligos but is especially designed for the annealing of DNA oligos used in RNAi (also known as siRNA) plasmid construction, which are often difficult to anneal. DNA oligos used for RNAi plasmid construction typically contain about 20 complementary sequences are and prone to forming hairpin structures, which can interfere with the correct annealing of the two DNA oligos. Using Aladdin's Annealing Buffer for DNA Oligos (10X) can effectively prevent the formation of hairpin structures in DNA oligos, and the annealed oligos can be directly ligated to purified plasmids that have been digested with restriction enzymes. This typically results in a high yield of positive clones after transformation. The operation with this product is very simple; just mix the DNA oligos to be annealed with the Annealing Buffer for DNA Oligos (10X) in a certain proportion and place the mixture in a PCR machine. The annealing process can be completed in about 20 minutes.
Precautions:
The Annealing Buffer for DNA Oligos (10X) is only suitable for the annealing of DNA oligos and should not be used for the annealing of RNA oligos.
This product is intended for research use by professionals only. It is not to be used for clinical diagnosis or treatment, nor is it to be used for food or pharmaceutical purposes. It should not be stored in ordinary residential areas.
For your safety and health, please wear a lab coat and disposable gloves when operating.
Specifications
Specifications & Purity
BioReagent, PCR Reagent, Suitable for molecular biology, sterile, for DNA and RNA applications, 10X
Stability And Storage
Store at -20 ℃. Upon receipt, it is recommended to aliquot. Avoid freeze/thaw cycle.
Storage
Store at -20°C, Avoid repeated freezing and thawing
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Grade
BioReagent, for DNA and RNA applications, PCR Reagent, Sterile, Suitable for molecular biology
Names and Identifiers
PH
8
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
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Products are supplied to verified businesses, institutions, and qualified professionals for research and development use only. Not for use in humans, animals, diagnosis, or therapy.
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