β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

CAS: 9001-45-0 Cat. No.: G755247 EC Number: 232-606-8

GRADE & PURITY Type H-5, lyophilized powder,≥400,000 units/g solid

Accession #

P09811

Overview

β-Glucuronidase Type H-2 from Helix pomatia is a crude solution of enzymes derived from the Roman snail. Many β-glucuronidases derived from mollusks also contain sulfatase activity.

Application:

β-Glucuronidase from Helix pomatia has been used:

• in the enzymatic treatment of urine samples for liquid chromatography-electrospray ionization-tandem mass spectrometry (LC-ESI-MS/MS) analysis
• in the quercetin extraction procedure from pig or rat tissues for high performance liquid chromatography (HPLC) analysis
• in the enzymatic deconjugation step in urine and plasma sample preparation for gas chromatography-mass spectrometry analysis (GC-MS)

β-glucuronidase was used to hydrolyze conjugated glucuronide and sulfate metabolites.

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Specifications

Product Name

β-Glucuronidase from Helix pomatia, CAS No.9001-45-0

Specifications & Purity

Type H-5, lyophilized powder, ≥400, 000 units/g solid

Biochemical and Physiological Mechanisms

β-glucuronidase (β-GIc) is an exoglycosidase that catalyzes the breakdown of complex carbohydrates. In humans it converts conjugated bilirubin into the unconjugated form, making bilirubin suitable for reabsorption.Used for the hydrolysis of glucuronide co

Accession #

P09811

CAS

9001-45-0

Enzyme Commission Number

3.2.1.31(BRENDA, IUBMB)

Molecule Type

Enzyme

Storage and Shipping

Concentration

Type H-5, lyophilized powder,≥400,000 units/g solid

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

References

1. Bairen Du, Jingjing Zhang, Yanjie Dong, Junwei Wang, Longwen Lei, Rengfei Shi.  (2020)  Determination of testosterone/epitestosterone concentration ratio in human urine by capillary electrophoresis.  STEROIDS,      [PMID:32603755] [10.1016/j.steroids.2020.108691]

2. Di Wu, Na Hu, Jichao Liu, Guangsen Fan, Xiuting Li, Jing Sun, Chunji Dai, Yourui Suo, Guoliang Li, Yongning Wu.  (2018)  Ultrasensitive colorimetric sensing strategy based on ascorbic acid triggered remarkable photoactive-nanoperoxidase for signal amplification and its application to α-glucosidase activity detection.  TALANTA,      [PMID:30172485] [10.1016/j.talanta.2018.07.073]