10mM in DMSO for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Store at -80°C Ships Dry ice packs + Cold packs Check lot-specific COA for exact specifications.
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Cited in 25 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Information
Bleomycin Sulfate (NSC125066) is a glycopeptide antibiotic and an anticancer agent for squamous cell carcinomas (SCC) with IC50 of 4 nM in UT-SCC-19A cells.
In vitro
UT-SCC-12A and UT-SCC-12B are both more resistant to Bleomycin sulfate with IC50 of 14.2 nM and 13 nM, respectively. Alveolar macrophages incubated with 0.01 μg/mL to 1μg /mL Bleomycin sulfate for 18 hours secretes significantly more fibroblast growth factor than macrophages incubated without Bleomycin sulfate. Macrophages stimulated with Bleomycin sulfate continues to produce significant amounts of fibroblast growth factor even after Bleomycin sulfate is removed and replaced with fresh (Bleomycin sulfate-free) media. Fibroblast growth factor secretion by Bleomycin sulfate-stimulated alveolar macrophages is inhibited by cycloheximide, and the 5-lipoxygenase inhibitors NDGA (nordihydroguaiaretic acid) and BW755c, indicating not only a requirement for protein synthesis but also for metabolites of the 5-lipoxygenase pathway of arachidonic acid metabolism for full expression of activity. Bleomycin sulfate (400 µg/mL) incubation for 24 hours decreases the viability of NTera-2 cells, and increases caspase-3, -8 and -9 activities, Bax and cytoplasmic cytochrome c levels and decreases Bcl-2 levels. In terms of unstable aberrations, the clastogenic effect of Bleomycin sulfate on ADIPO-P2 cells persists for at least 10 days after exposure. Bleomycin sulfate-induced telomere instability in mammalian cells persists for several generations after exposure. Moreover, the appearance of telomere fusions in Bleomycin sulfate-exposed cells 10 days after treatment suggests that Bleomycin sulfate can induce delayed telomere instability.
In vivo
Day 7 post-Bleomycin sulfate, CD45+ cells in BALf in NOX-/- is 1.7-fold > WT, 57% of which are Mf that decreases by 67% in WT and 83% in NOX-/- by Day 21.
Cell Data
cell lines：
Concentrations：2.5 μg/mL
Incubation Time：30 minutes
Powder Purity:≥98%
| Isomeric SMILES | CC1=C(N=C(N=C1N)C(CC(=O)N)NCC(C(=O)N)N)C(=O)NC(C(C2=CN=CN2)O[C@H]3[C@H]([C@H]([C@@H]([C@@H](O3)CO)O)O)O[C@@H]4[C@H]([C@H]([C@@H]([C@H](O4)CO)O)OC(=O)N)O)C(=O)NC(C)C(C(C)C(=O)NC(C(C)O)C(=O)NCCC5=NC(=CS5)C6=NC(=CS6)C(=O)NCCC[S+](C)C)O.OS(=O)(=O)[O-] |
|---|---|
| WGK Germany | 3 |
| RTECS | EC5991990 |
| Reaxy-Rn | 24931218 |
| Reaxys-RN_link_address | https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=24931218&ln= |
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →Full quality attributes and acceptance criteria for this grade.
View spec sheet →| 1. Lijun Fang, Linmao Lyu, Hongyu Zhong, Jiamao Lin, Pengtao Ren, Yu Wang, Yunhong Yin, Mengyu Zhang, Yiqing Qu. (2025) Transcription Factor MEOX1 Accelerates Pulmonary Fibrosis by Regulating Mitophagy and Senescence. EUROPEAN JOURNAL OF PHARMACOLOGY, [PMID:40780596] [10.1016/j.ejphar.2025.178043] |
| 2. La Yan, Juanyu Zhou, Lu Yuan, Jinbao Ye, Xudong Zhao, Gang Ren, Haiyang Chen. (2023) Silibinin alleviates intestinal inflammation via inhibiting JNK signaling in Drosophila. Frontiers in Pharmacology, [PMID:37781701] [10.3389/fphar.2023.1246960] |
| 3. Jing Chang, Jiahui Wang, Beibei Luo, Weihao Li, Ziyue Xiong, Chaoqi Du, Xue Wang, Yuejiao Wang, Jingya Tian, Shuxin Li, Yue Fang, Longjie Li, Jing Dong, Ke Tan, Yumei Fan, Pengxiu Cao. (2023) Vitamin E stabilizes iron and mitochondrial metabolism in pulmonary fibrosis. Frontiers in Pharmacology, [PMID:38125893] [10.3389/fphar.2023.1240829] |
| 4. La Yan, Xiaoxin Guo, Juanyu Zhou, Yuedan Zhu, Zehong Zhang, Haiyang Chen. (2022) Quercetin Prevents Intestinal Stem Cell Aging via Scavenging ROS and Inhibiting Insulin Signaling in Drosophila. Antioxidants, 12 (1): (59). [PMID:36670921] [10.3390/antiox12010059] |
| 5. Liu Yunzhi, Xie Xianghuan, Wang Ping, Luo Jialiang, Chen Yu, Xu Qishan, Zhou Jia, Lu Xiao, Zhao Jianbo, Chen Zhengliang, Zuo Daming. (2022) Mannan-Binding Lectin Reduces Epithelial-Mesenchymal Transition in Pulmonary Fibrosis via Inactivating the Store-Operated Calcium Entry Machinery. Journal of Innate Immunity, 15 (1): (37-49). [PMID:35671705] [10.1159/000524693] |
| 6. Deliang Zhang, Rongqiang Zhuang, Jindian Li, Yuting Lv, Xia Yang, Weimin Pan, Xianzhong Zhang. (2021) MicroSPECT Imaging-Guided Treatment of Idiopathic Pulmonary Fibrosis in Mice with a Vimentin-Targeting 99mTc-Labeled N-Acetylglucosamine-Polyethyleneimine. MOLECULAR PHARMACEUTICS, [PMID:34657437] [10.1021/acs.molpharmaceut.1c00545] |
| 7. Lijun Fang, Wei Wang, Jiazheng Chen, Anju Zuo, Hongmei Gao, Tao Yan, Pengqi Wang, Yujia Lu, Ruijuan Lv, Feng Xu, Yuguo Chen, Linmao Lyu. (2021) Osthole Attenuates Bleomycin-Induced Pulmonary Fibrosis by Modulating NADPH Oxidase 4-Derived Oxidative Stress in Mice. Oxidative Medicine and Cellular Longevity, [PMID:34527170] [10.1155/2021/3309944] |
| 8. Xue Liu, Yumeng Shao, Xinyue Zhang, Xiang Ji, Min Xie, Huaman Liu. (2021) Calycosin attenuates pulmonary fibrosis by the epithelial-mesenchymal transition repression upon inhibiting the AKT/GSK3β/β-catenin signaling pathway. ACTA HISTOCHEMICA, [PMID:34217047] [10.1016/j.acthis.2021.151746] |
| 9. Gang Du, Zhiming Liu, Zihua Yu, Zhangpeng Zhuo, Yuedan Zhu, Juanyu Zhou, Yang Li, Haiyang Chen. (2021) Taurine represses age-associated gut hyperplasia in Drosophila via counteracting endoplasmic reticulum stress. AGING CELL, 20 (3): (e13319). [PMID:33559276] [10.1111/acel.13319] |
| 10. Hongjin Chen, Yali Zhang, Wenxin Zhang, Hui Liu, Chuchu Sun, Bing Zhang, Bin Bai, Di Wu, Zhongxiang Xiao, Hazel Lum, Jianmin Zhou, Ruijie Chen, Guang Liang. (2019) Inhibition of myeloid differentiation factor 2 by baicalein protects against acute lung injury. PHYTOMEDICINE, [PMID:31254764] [10.1016/j.phymed.2019.152997] |
| 11. Qiu-Ling Li, Xin Chang, Yu-Mo Han, Zi-Chao Guo, Yi-Na Liu, Bin Guo, Chang Liu, Bin-Rong Yang, Zhong-Kai Fan, Hu-Lin Jiang, Xin Chang. (2024) Consumption of Endogenous Caspase-3 Activates Molecular Theranostic Nanoplatform against Inflammation-Induced Profibrotic Positive Feedback in Pulmonary Fibrosis. Advanced Science, [PMID:39686776] [10.1002/advs.202412303] |
| 12. Wen-Jing Zhong, Chen-Yu Zhang, Jia-Xi Duan, Meng-Rui Chen, Ping-Deng, Bo-Liang Zhang, Nan-Shi-Yu Yang, Han-Xi Sha, Jun Zhang, Jian-Bing Xiong, Cha-Xiang Guan, Yong Zhou. (2024) Krüppel-like transcription factor 14 alleviates alveolar epithelial cell senescence by inhibiting endoplasmic reticulum stress in pulmonaryfibrosis. INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES, [PMID:39270890] [10.1016/j.ijbiomac.2024.135351] |
| 13. Heting Zhou, Xinyue Zheng, Shaolin Huang, Xiaomeng Wang, Ting Zhou, Shuwen Zhang, Yihan Ling, Wenxi Wang, Xingjie Li, Shouqian Li, Yongmei Xie, Wenya Yin. (2025) Rosa roxburghii Fermentation Broths Attenuate Bleomycin-Induced Pulmonary Fibrosis by Activating the Nrf2/HO-1/NQO1 Signaling Pathway and Modulating Gut Microbiota. Food Science & Nutrition, 13 (3): (e70105). [PMID:40115251] [10.1002/fsn3.70105] |
| 14. Xiaomeng Wang, Ting Zhou, Shaolin Huang, Heting Zhou, Yihan Ling, Tao Chen, Shuwen Zhang, Wenxi Wang, Chuan Wu, Wenya Yin. (2025) Screening and validation of active components in Rosa roxburghii Tratt for anti-pulmonary fibrosis based on a spectrum-effect relationship. INTERNATIONAL IMMUNOPHARMACOLOGY, [PMID:40154178] [10.1016/j.intimp.2025.114536] |
| 15. Li Zhipeng, Jiao Yang, Wu Zhisong, Liu Haoge, Li Yang, Cai Yaodong, Wei Wan, Cao Fang. (2024) The role of quercetin in ameliorating bleomycin-induced pulmonary fibrosis: insights into autophagy and the SIRT1/AMPK signaling pathway. MOLECULAR BIOLOGY REPORTS, 51 (1): (1-10). [PMID:39001907] [10.1007/s11033-024-09752-7] |
| 16. Ying Liu, Xinran Wang, Chenrong Jin, Juhui Qiao, Chenxi Wang, Leilei Jiang, Shiting Yu, Daian Pan, Daqing Zhao, Siming Wang, Meichen Liu. (2024) Total ginsenosides extend healthspan of aging Drosophila by suppressing imbalances in intestinal stem cells and microbiota. PHYTOMEDICINE, [PMID:38669971] [10.1016/j.phymed.2024.155650] |
| 17. Xiaoxin Guo, Juanyu Zhou, La Yan, Xingzhu Liu, Yu Yuan, Jinbao Ye, Zehong Zhang, Haiou Chen, Yongxin Ma, Zhendong Zhong, Guanzheng Luo, Haiyang Chen. (2024) Very long-chain fatty acids control peroxisome dynamics via a feedback loop in intestinal stem cells during gut regeneration. DEVELOPMENTAL CELL, [PMID:39047737] [10.1016/j.devcel.2024.06.020] |
| 18. Zhong Wen-Jing, Xiong Jian-Bing, Zhang Chen-Yu, Jin Ling, Yang Nan-Shi-Yu, Sha Han-Xi, Liu Yu-Biao, Duan Jia-Xi, Guan Cha-Xiang, Zhou Yong, Su Feng. (2025) Blocking triggering receptors expressed on myeloid cell-1 alleviates alveolar epithelial cell senescence by inhibiting oxidative stress in pulmonary fibrosis. HISTOCHEMISTRY AND CELL BIOLOGY, 163 (1): (1-15). [PMID:40240638] [10.1007/s00418-025-02374-5] |
| 19. Jinhua Yan, Chenxi Feng, Hanmei Zhang, Ting Luo, Haiyang Chen, Haiou Chen. (2025) Dapagliflozin ameliorates intestinal stem cell aging by regulating the MAPK signaling pathway in Drosophila. Frontiers in Cell and Developmental Biology, [PMID:40337552] [10.3389/fcell.2025.1576258] |
| 20. Xue Wang, Xin Liu, Yumei Fan, Ke Tan, Jiaqi Gao, Yuejiao Wang, Ziyi Zhang, Shuyue Liu, Xiaofan Wang, Ke Tan, Baohua Wang, Pengxiu Cao. (2025) FOXF1 and SHH participate in the regulation of iron signaling in pulmonary fibrosis. Redox Biology, [PMID:41101211] [10.1016/j.redox.2025.103893] |
| 21. Ruixi Luo, Yaqiong Wei, La Wang, Peng Chen, Didong Lou, Weiyi Tian. (2025) Targeting ER stress-mediated apoptosis by MSC-derived exosomes: A novel therapeutic strategy against pulmonary fibrosis. Regenerative Therapy, [PMID:41147010] [10.1016/j.reth.2025.10.004] |
| 22. Li Zhipeng, Liu Zhaoheng, Wei Wan, Niu Jie, Wu Jianjun, Jiao Yang. (2025) Improvement of Number 2 Feibi Recipe on pulmonary fibrosis in mice by inhibiting the level of miR-199a-5p and activating autophagy. Scientific Reports, 15 (1): (44020). [PMID:41408064] [10.1038/s41598-025-24453-2] |
| 23. Lu Pu, Zhiliang Zuo, Hui Zheng, Rui Ou, Ru Gao, Zhaomin Deng, Xiaochu Wu, Chun Xiao, Meiling Ge, Lixing Zhou, Haoran Jin, Shaochong Qi, Fengjuan Hu, Jieli Chen, Hang Li, Yan Zhao, Birong Dong, Hao Jiang. (2026) ERG Phase Separation Attenuates Cellular Senescence. iScience, [PMID:41704762] [10.1016/j.isci.2026.114678] |
| 24. Ce Li, Yichao Yuan, Yaqing Bao, Yeling Ni, Nan Chen, Jigang Piao, Lu Wang, Zhihong Zhu. (2026) Synergistic regulation of TGF-β1/Smad2/3 signaling and inflammatory pathways via SA/NAC-based nanoplatforms: a novel strategy to enhance anti-fibrotic therapeutic outcomes in idiopathic pulmonary fibrosis. JOURNAL OF NANOBIOTECHNOLOGY, [PMID:41582130] [10.1186/s12951-026-04071-4] |
| 25. An Chang, Yi Wang, Zan Cui, Yuan-Yuan Liu, Ling-Qi Meng, Jia-Shi, Zhi-Yong Qin, Jia-Ning Gong. (2026) Dual-drug nanoplatform HPA@NPs reprograms the fibrotic microenvironment by suppressing EMT and M2 macrophage polarization in pulmonary fibrosis. INTERNATIONAL IMMUNOPHARMACOLOGY, [PMID:41679177] [10.1016/j.intimp.2026.116362] |
