Carboxypeptidase G, CAS No.9074-87-7

CAS: 9074-87-7 Cat. No.: C755300 EC Number: 232-978-1 PubChem CID: 21195079

GRADE & PURITY Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥10 U/mg enzyme powder；≥50 U/mg protein; expressed in E.coli

Accession #

P06621

Expression system

E. coli

Bioactivity

≥10 U/mg enzyme powder；≥50 U/mg protein

Overview

  Carboxypeptidase G or γ-Glutamyl hydrolase, has been used in a study to assess the role of the putidaredoxin COOH-terminus in P-450cam (cytochrome m) hydroxylations. Carboxypeptidase G from Pseudomonas sp. has also been used in a study to investigate the effects of nitric oxide on pemetrexed cytotoxicity via NO‑cGMP signaling in lung adenocarcinoma cells.

Specifications

Product Name

Carboxypeptidase G, CAS No.9074-87-7

Synonyms

carboxypeptidase G1 | Carboxypeptidase G2 | glutamate carboxypeptidase | γ-Glutamyl hydrolase | cpg2

Grade

ActiBioPure™, Bioactive, High Performance, Recombinant, EnzymoPure™

Specifications & Purity

Bioactive, Recombinant, ActiBioPure™, High Performance, EnzymoPure™, ≥90%(SDS-PAGE), ≥10 U/mg enzyme powder；≥50 U/mg protein; expressed in E.coli

Biochemical and Physiological Mechanisms

Catalyzes the hydrolysis of reduced and non-reduced folates to pteroates and L-glutamate. This enzyme has a broad specificity. Catalytic activity: Release of C-terminal glutamate residues from a wide range of N-acylating moieties, including peptidyl, amin

Bioactivity

≥10 U/mg enzyme powder；≥50 U/mg protein

Accession #

P06621

CAS

9074-87-7

Enzyme Commission Number

EC 3.4.17.11

Molecule Type

Enzyme

Storage and Shipping

Concentration

≥10 U/mg enzyme powder；≥50 U/mg protein; expressed in E.coli

Reconstitution

It is recommended to reconstitute the lyophilized powder in sterile 18M-cm H2O.

Storage

Store at 2-8°C

Shipped In

Wet ice

Stability And Storage

Store at 2-8℃ long term (12 months). Upon reconstitution, it is recommended to aliquot.

Unit definition

One unit will convert methotrexate to 1.0 μmole L-glutamic acid per minute at pH7.3 at 30 centigrade.

Taxonomic Classification

Taxonomy Tree

• Kingdom Inorganic compounds
  • Superclass Homogeneous metal compounds
    • Class Homogeneous transition metal compounds

• Kingdom: Inorganic compounds
• Superclass: Homogeneous metal compounds
• Class: Homogeneous transition metal compounds
• Subclass: Not available
• Intermediate Tree Nodes: Not available
• Direct Parent: Homogeneous transition metal compounds
• Alternative Parents: Not available
• Molecular Framework: Not available
• Substituents: Homogeneous transition metal
• Description: This compound belongs to the class of inorganic compounds known as homogeneous transition metal compounds. These are inorganic compounds containing only metal atoms,with the largest atom being a transition metal atom.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y