CD31 Rat mAb (AF488) - Primary antibody, specific to CD31

Cat. No.: Ab190227

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

390

Conjugation

AF488

Overview

Rat anti Mouse CD31 Antibody, Monoclonal (390), could be used for Flow Cytometry.

Specifications

Product Name

CD31 Rat mAb (AF488) - Primary antibody, specific to CD31

Synonyms

Cd31 | Pecam | adhesion molecule | CD31 antigen | CD31/EndoCAM | EndoCAM | FLJ34100 | FLJ58394 | GPIIA' | PECA1 | PECAM1 | PECAM-1 | PECAM-1, CD31/EndoCAM | platelet endothelial cell adhesion molecule | platelet endothelial cell adhesion molecule-1 | plat

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Induces susceptibility to atherosclerosis (By similarity). Cell adhesion molecule which is required for leukocyte transendothelial migration (TEM) under most inflammatory conditions. Tyr-690 plays a critical role in TEM and is required for efficient traff

Host species

Rat

Specificity

CD31

Immunogen

C3H/HeJ mouse hematopoietic progenitor cell line 3

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

AF488

Excitation (nm)

Blue Laser(488nm)

Emission(nm)

525nm

Ex/Em(nm)

Ex:490nm;Em:525nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD31 Rat mAb (AF488) (Ab190227)

CD31 Rat mAb (AF488) (Ab190227) - Flow Cytometry
Flow cytometry analysis of CD31 expression by C57BL/6 mouse splenocytes (right panel) compared to an isotype control (left panel). Cells were stained with CD31 Rat mAb (AF488) (Ab190227) at 0.3μg/test for 1 hour at 4°C. Left panel - Rat IgG (AF488) (Ab190240).

Flow Cytometry: CD31 Rat mAb (AF488) (Ab190227)

CD31 Rat mAb (AF488) (Ab190227) - Flow Cytometry
Flow cytometry analysis of CD31 expression by C57BL/6 mouse splenocytes (red) compared to an isotype control (blue). Cells were stained with CD31 Rat mAb (AF488) (Ab190227) at 0.3μg/test for 1 hour at 4°C. Blue - Rat IgG (AF488) (Ab190240). Black - Unlabelled control, cells without incubation with primary antibody.

Application

• Application: Dilution info
• Flow Cytometry:

  ≤1.0μg/ test

  For flow cytometric staining, the suggested use of this reagent is ≤1.0 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

PECAM1 Tbio Platelet endothelial cell adhesion molecule (0 Activities)

Discover Target: PECAM1

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)