CD86 Rat mAb (PE-Cy7) - Primary antibody, specific to CD86

Cat. No.: Ab215327

GRADE & PURITY Validated, ExactAb™, 0.5 mg/mL

Application

Flow Cytometry

Host species

Rat

Reactive species

Mouse

Isotype

Rat IgG2a

Clone Number

GL1

Conjugation

PE-Cy7

Overview

Rat anti Mouse CD86 Antibody, Monoclonal (GL1), could be used for Flow Cytometry.

Specifications

Product Name

CD86 Rat mAb (PE-Cy7) - Primary antibody, specific to CD86

Specifications & Purity

ExactAb™, Validated, 0.5 mg/mL

Biochemical and Physiological Mechanisms

Receptor involved in the costimulatory signal essential for T-lymphocyte proliferation and interleukin-2 production, by binding CD28 or CTLA-4. May play a critical role in the early events of T-cell activation and costimulation of naive T-cells, such as d

Host species

Rat

Specificity

CD86

Immunogen

LPS-activated CBA/Ca mouse splenic B cells

Positive Control

Flow Cytometry: C57BL/6 mouse splenocytes

Conjugation

PE-Cy7

Excitation (nm)

Blue Laser(488nm), Green Laser(532nm), Yellow-Green Laser(561nm)

Emission(nm)

774nm

Ex/Em(nm)

Ex:565nm;Em:774nm

Grade

ExactAb™, Validated

Light Chain Type

kappa

Purification Method

Protein G purified

Concentration

0.5 mg/mL

Storage and Shipping

Storage

Store at 2-8°C,Protected from light,Do not freeze

Shipped In

Wet ice,Do not freeze

Stability And Storage

The antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light. Do not freeze.

Images

Flow Cytometry: CD86 Rat mAb (PE-Cy7) (Ab215327)

CD86 Rat mAb (PE-Cy7) (Ab215327) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes (right panel) were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD86 Rat mAb (PE-Cy7) (Ab215327) compared to an isotype control (blue). Blue - Rat IgG (PE-Cy7) (Ab190073). Black - Unlabelled control, cells without incubation with primary antibody. Untreated C57BL/6 mouse splenocytes were used as control (left panel).

Flow Cytometry: CD86 Rat mAb (PE-Cy7) (Ab215327)

CD86 Rat mAb (PE-Cy7) (Ab215327) - Flow Cytometry
LPS-stimulated (3 days) C57BL/6 mouse splenocytes were preincubated with CD16/CD32 Rat mAb (A412018). The cells were then stained with CD86 Rat mAb (PE-Cy7) (Ab215327) (right panel) at 0.1μg/test for 1 hour at 4°C. Left panel - Rat IgG (PE-Cy7) (Ab190073).

Application

• Application: Dilution info
• Flow Cytometry:

  ≤0.3μg/test

  For flow cytometric staining, the suggested use of this reagent is ≤0.3 μg per million cells in 100 µL staining volume.;

Associated Targets(Human)

CD86 Tclin T-lymphocyte activation antigen CD86 (0 Activities)

Discover Target: CD86

Activity Type	Activity Value -log(M)	Mechanism of Action	Activity Reference	Publications (PubMed IDs)