This series of Cell Cryopreservation Medium is developed in layers to meet diverse research demands, covering three product models: C1525335, C1525336 and C1525338. It covers three application scenarios including cost-effective routine cell culture, high-standard serum-free protein-based cryopreservation and dedicated low-temperature storage for red blood cells, compatible with most mainstream in vitro experimental samples in laboratories.
Product No.
| Product Name | Core Components | Product Features | Suitable Cell Lines | | C1525335 | Cell Cryopreservation Medium (Serum-Free, with Protein) | Amino acids, inorganic salts, albumin, and cryoprotectants
| Medicinal-grade human-derived protein is used to replace traditional serum, which not only avoids the risk of exogenous virus contamination, but also completely eliminates the impact of serum batch variation on experimental results; meanwhile, it is compounded with cell membrane protectant of optimized concentration, which can greatly improve cell viability. | 293T, B16-F10, Hela, HepG2, HT22, 4T1, MDA-MB-231, DC2.4, H9C2, ST, etc. | | C1525336 | Cell Cryopreservation Medium (Protein-Free, Serum-Free) | Amino acids, inorganic salts, and cryoprotectants
| Routine DMSO ratio with high cost performance. | L929, K562, HT22, etc. | | C1525338 | RBC Cryopreservation Medium
| lycerol, Cryoprotectant, Normal saline, etc. without DMSO
| Protects red blood cells from ice crystal damage during low-temperature freezing, maintains the integrity and function of cell membranes, and provides necessary nutrients to support short-term cell survival. | Red Blood Cells |
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Main Components: Amino acids, inorganic salts, albumin, cryoprotectants.
Product Positioning: Suitable for primary cells and various tumor cells, capable of retaining cell functions and proliferation rate of tumor cells after resuscitation.
Intended Use: For cryopreservation of various cells. Cryopreserved cells are only for scientific research or in vitro analytical testing, and cannot be used for clinical treatment.
Instructions for Use: This product is sterilized before filling and can be used without dilution; cell culture shall be carried out in accordance with corresponding operating procedures.
Cell Cryopreservation Protocol:
1.Harvest cells in logarithmic growth phase, centrifuge at 1000 rpm for 5 min and discard the supernatant.
2.Resuspend cells with complete medium for cell counting and viability detection, then centrifuge and remove the supernatant (This step can be omitted if cell counting and initial cell status observation are unnecessary).
3.Resuspend cells with this product (pipette gently to avoid air bubbles). The recommended cryopreservation density is 1×10⁶-1×10⁷ cells/mL, with 0.5–1.5 mL medium per cryovial.
4.Incubate dispensed cryovials at 4 ℃ for 20 min, then transfer to -80 ℃ refrigerator for overnight storage, and move into liquid nitrogen for preservation the next day.
Notes:
[1] Pre-cool the product in a 4 ℃ refrigerator before use to prevent cell damage caused by high temperature of cryoprotectants.
[2] Make sure cryovials are completely sealed before placing into liquid nitrogen to avoid tube rupture during resuscitation.
Cell Thawing Protocol:
1.Take the cryovial with cells out of liquid nitrogen and rapidly thaw in a 37 ℃ water bath.
2.Transfer thawed cell suspension into a sterile centrifuge tube with equal volume of complete medium, centrifuge at 1000 rpm for 5 min and discard supernatant, then resuspend cells with complete medium.
3.Transfer the above cell suspension into a cell culture flask filled with complete medium, mix evenly and culture in cell incubator.
Notes: Test results detected immediately after resuscitation may be inaccurate; detection after cell attachment or 4–48 h (adjust according to cell growth characteristics) will be more accurate.
General Precautions:
1.Transfer the product to a 4 ℃ refrigerator immediately after receiving.
2.In case of skin or eye contact during use, rinse with plenty of clean water at once, and seek medical treatment if discomfort occurs.
3.Do not use the product if turbidity, precipitation or other deterioration appears, and contact technical personnel immediately.
4.Open the bottle cap inside a biosafety cabinet to avoid cell contamination.
