CSPD - Moligand™,≥95% , CAS No.142456-88-0

CAS: 142456-88-0 Cat. No.: C1510850 Molecular Weight: 416.79

GRADE & PURITY Moligand™, ≥95%

Synonyms

3-(2'-(spiro-5-chloroadamantane))-4-methoxy-4-(3''-phosphoryloxy)phenyl-1,2-dioxetane

Storage

Protected from light,Store at -20°C,Argon charged

Shipped In

Ice chest + Ice pads

Overview

CSPD is a chemiluminescent substrate for alkaline phosphatase, used for protein or nucleic acid blotting on nitrocellulose membranes. These substrates can also be employed in solution-based assays. The CSPD chemiluminescent substrate enables the detection of alkaline phosphatase and alkaline phosphatase-labeled molecules with exceptional sensitivity, speed, and ease of use. It serves as a highly sensitive alternative to widely used fluorescent substrates like methylumbelliferyl phosphate (MUP) and chromogenic substrates such as p-nitrophenyl phosphate (pNPP). The combination of low background luminescence and high-intensity light output allows for the detection of alkaline phosphatase-labeled molecules with the highest possible sensitivity and signal-to-noise ratio. This versatile chemiluminescent substrate demonstrates high sensitivity in membrane-based applications, such as Southern, Northern, and Western blotting. It is also suitable for solution-based assays, including immunoassays, DNA probe tests, enzyme assays, and reporter gene detection. Maximum luminescence is reached within approximately 10 minutes, with light emission persisting for several hours. Solution-based assays require a chemiluminescence enhancer, while membrane-based assays may also need this reagent to enhance light output and sensitivity.

Specifications

Synonyms

3-(2'-(spiro-5-chloroadamantane))-4-methoxy-4-(3''-phosphoryloxy)phenyl-1, 2-dioxetane

Specifications & Purity

Moligand™, ≥95%

Storage

Protected from light, Store at -20°C, Argon charged

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.

Grade

Moligand™

Purity

≥95%

Names and Identifiers

• Canonical Smiles: COC1(C2(C3CC4CC2CC(C4)(C3)Cl)OO1)C5=CC(=CC=C5)OP(=O)(O)O
• IUPAC Name: [3-(1-chloro-3'-methoxyspiro[adamantane-4,4'-dioxetane]-3'-yl)phenyl] dihydrogen phosphate
• InChIKey: QWXOJIDBSHLIFI-UHFFFAOYSA-N
• INCHI: 1S/C18H22ClO7P/c1-23-18(12-3-2-4-15(7-12)24-27(20,21)22)17(25-26-18)13-5-11-6-14(17)10-16(19,8-11)9-13/h2-4,7,11,13-14H,5-6,8-10H2,1H3,(H2,20,21,22)
• Molecular Weight: 416.79

Taxonomic Classification

Taxonomy Tree

• Kingdom Organic compounds
  • Superclass Organic acids and derivatives
    • Class Organic phosphoric acids and derivatives
      • Subclass Phosphate esters
        • Level5 Aryl phosphates
          • Level6 Aryl phosphomonoesters
            • Level7 Phenyl phosphates

• Kingdom: Organic compounds
• Superclass: Organic acids and derivatives
• Class: Organic phosphoric acids and derivatives
• Subclass: Phosphate esters
• Intermediate Tree Nodes: Aryl phosphates - Aryl phosphomonoesters
• Direct Parent: Phenyl phosphates
• Alternative Parents: Benzylethers Phenoxy compounds Dioxetanes Dialkyl peroxides Oxacyclic compounds Organooxygen compounds Organochlorides Hydrocarbon derivatives Alkyl chlorides
• Molecular Framework: Aromatic heteropolycyclic compounds
• Substituents: Phenyl phosphate - Benzylether - Phenoxy compound - Monocyclic benzene moiety - Benzenoid - Dialkyl peroxide - 1,2-dioxetane - Oxacycle - Organoheterocyclic compound - Alkyl halide - Organooxygen compound - Organochloride - Organohalogen compound - Organic oxygen compound - Alkyl chloride - Hydrocarbon derivative - Organic oxide - Aromatic heteropolycyclic compound
• Description: This compound belongs to the class of organic compounds known as phenyl phosphates. These are aromatic organooxygen compounds containing a phosphate group, which is O-esterified with a phenyl group.
• External Descriptors: Not available

Data sources

1. Djoumbou Feunang Y, Eisner R, Knox C, Chepelev L, Hastings J, Owen G, Fahy E, Steinbeck C, Subramanian S, Bolton E, Greiner R, and Wishart DS. ClassyFire: Automated Chemical Classification With A Comprehensive, Computable Taxonomy. Journal of Cheminformatics, 2016, 8:61.

DOI: 10.1186/s13321-016-0174-y

Chemical and Physical Properties

• Sensitivity: Moisture sensitive
• Molecular Weight: 416.800 g/mol
• XLogP3: 1.800
• Hydrogen Bond Donor Count: 2
• Hydrogen Bond Acceptor Count: 7
• Rotatable Bond Count: 4
• Exact Mass: 416.079 Da
• Monoisotopic Mass: 416.079 Da
• Topological Polar Surface Area: 94.500 Ų
• Heavy Atom Count: 27
• Formal Charge: 0
• Complexity: 652.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 3
• Defined Bond Stereocenter Count: 0
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 0
• Covalently-Bonded Unit Count: 1