DOTAP Transfection Reagent - 1 mg/ml in MES-buffered saline , CAS No.144189-73-1

CAS: 144189-73-1 Cat. No.: D353304 Molecular Weight: 774.19

GRADE & PURITY 1 mg/ml in MES-buffered saline

Synonyms

DOTAP | 18:1 TAP (DOTAP, MS Salt), 1,2-dioleoyl-3-trimethylammonium-propane (methyl sulfate salt), chloroform | N,N,N-Trimethyl-2,3-bis(oleoyloxy)propan-1-aminium methyl sulfate | DOTAP Liposomal Transfection Reagent, N-[1-(2,3-Dioleoyloxy)propyl]-N,N,N-t

Storage

Store at 2-8°C

Shipped In

Wet ice

Overview

DOTAP Transfection Reagent is a liposomal formulation of the cationic lipid DOTAP. DOTAP Transfection Reagent is used not only for DNA and RNA transfection, but yeast artificial chromosomes (YAC) as well, with or without serum. This transfection via DOTAP Transfection Reagent is between eukaryotic cells in iehter transient or stable gene expression. DOTAP Transfection Reagent is also suitable for other negatively charged molecules, including oligonucleotidees and glutamic acid.

Specifications

Synonyms

DOTAP | 18:1 TAP (DOTAP, MS Salt), 1, 2-dioleoyl-3-trimethylammonium-propane (methyl sulfate salt), chloroform | N, N, N-Trimethyl-2, 3-bis(oleoyloxy)propan-1-aminium methyl sulfate | DOTAP Liposomal Transfection Reagent, N-[1-(2, 3-Dioleoyloxy)propyl]-N, N, N-t

Specifications & Purity

1 mg/ml in MES-buffered saline

Storage

Store at 2-8°C

Shipped In

Wet ice

This product requires cold chain shipping. Ground and other economy services are not available.

Names and Identifiers

• Canonical Smiles: CCCCCCCCC=CCCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCCC=CCCCCCCCC.COS(=O)(=O)[O-]
• IUPAC Name: 2,3-bis[[(Z)-octadec-9-enoyl]oxy]propyl-trimethylazanium;methyl sulfate
• InChIKey: RSMRWWHFJMENJH-LQDDAWAPSA-M
• INCHI: 1S/C42H80NO4.CH4O4S/c1-6-8-10-12-14-16-18-20-22-24-26-28-30-32-34-36-41(44)46-39-40(38-43(3,4)5)47-42(45)37-35-33-31-29-27-25-23-21-19-17-15-13-11-9-7-2;1-5-6(2,3)4/h20-23,40H,6-19,24-39H2,1-5H3;1H3,(H,2,3,4)/q+1;/p-1/b22-20-,23-21-
• Isomeric SMILES: CCCCCCCC/C=C\CCCCCCCC(=O)OCC(C[N+](C)(C)C)OC(=O)CCCCCCC/C=C\CCCCCCCC.COS(=O)(=O)[O-]
• WGK Germany: 3
• Molecular Weight: 774.19
• Reaxy-Rn: 34197390
• Reaxys-RN_link_address: https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=34197390&ln=

Chemical and Physical Properties

• Boil Point(°C): 100 °C
• Melt Point(°C): -5 °C
• Molecular Weight: 774.200 g/mol
• Hydrogen Bond Donor Count: 0
• Hydrogen Bond Acceptor Count: 8
• Rotatable Bond Count: 37
• Exact Mass: 773.584 Da
• Monoisotopic Mass: 773.584 Da
• Topological Polar Surface Area: 127.000 Ų
• Heavy Atom Count: 53
• Formal Charge: 0
• Complexity: 845.000
• Isotope Atom Count: 0
• Defined Atom Stereocenter Count: 0
• Undefined Atom Stereocenter Count: 1
• Defined Bond Stereocenter Count: 2
• Undefined Bond Stereocenter Count: 0
• The total count of all stereochemical bonds: 2
• Covalently-Bonded Unit Count: 2

XLogP3

References

1. Yandong Xie, Xueying Lu, Zhen Wang, Mingxi Liu, Liang Liu, Ran Wang, Kun Yang, Hong Xiao, Jianyong Li, Xianglong Tang, Hongyi Liu.  (2022)  A hypoxia-dissociable siRNA nanoplatform for synergistically enhanced chemo-radiotherapy of glioblastoma.  Biomaterials Science,  10  (23): (6791-6803).  [PMID:36314541] [10.1039/D2BM01145J]

2. Binke Wang, Xinyu Qi, Ruping Yang, Xu Xu, Shiyao Li, Chunli Zhang, Haitao Yu.  (2025)  Cubosome-based co-delivery of doxorubicin and polydatin for targeted cancer therapy.  CHEMICAL ENGINEERING JOURNAL,      [PMID:] [10.1016/j.cej.2025.168855]