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DL 405 Maleimide , CAS No.D638911
AVAILABLE TO ORDER
Storage
Store at -20°C,Desiccated
Shipped In
Ice chest + Ice pads
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Why this grade
for sensitive chromatographic and analytical workflows requiring minimal baseline interference.
Storage & shipping
Store at -20°C,Desiccated Ships Ice chest + Ice pads Check lot-specific COA for exact specifications.
Quality documents
SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.
Literature proof
Cited in 0 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.
Overview
DyLight 405 Sulfhydryl-Reactive Dye is a maleimide-activated derivative of high-performance DyLight 405 used to fluorescently label sulfhydryl-containing peptides, proteins and other biomolecular probes.
has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than AF 405, Cascade™ Blue and AMCA dyes in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.
Features of DyLight 405 Maleimide:
• High performance—DyLight 405 shows brighter fluorescence than AF405, Cascade Blue and AMCA
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
• Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
• Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency
Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
DyLight 405 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.
has high fluorescence intensity over a broad pH range (pH 4-9) and is more photostable than AF 405, Cascade™ Blue and AMCA dyes in many applications. The high water solubility of DyLight Fluors means that a high dye-to-protein ratio can be attained without causing precipitation of the conjugates.
Features of DyLight 405 Maleimide:
• High performance—DyLight 405 shows brighter fluorescence than AF405, Cascade Blue and AMCA
• Specific—maleimide-activated dye labels proteins and other molecules at reduced sulfhydryls (-SH)
• Efficient labeling methods—well-characterized chemistry and optimized protocols provide for reliable, high-quality labeling
• Optimized antibody labeling procedure—complete protocol for IgG reduction and labeling and calculating the labeling efficiency
Applications:
• Antibody labeling for immunofluorescence applications, including immunocytochemistry (ICC), immunohistochemistry (IHC), Western blotting and ELISA assay
• Target macromolecule labeling for in vitro and in vivo fluorescent detection strategies
DyLight 405 Sulfhydryl-Reactive Dye is activated with a maleic acid imide (maleimide) moiety to form a reactive alkylation reagent. Labeling occurs through reaction of the maleimide-activated dye with reduced sulfhydryl groups (-SH) to form stable thioether bonds. Maleimides are specific for sulfhydryl groups between pH 6.5-7.5. Learn more about maleimide chemistry.
Specifications
Storage
Store at -20°C, Desiccated
Shipped In
Ice chest + Ice pads
This product requires cold chain shipping. Ground and other economy services are not available.
Product Properties
| Reactivity | Thiol |
|---|
Documentation
📋 Safety Data Sheet (SDS)
Comprehensive hazard, handling, storage, and regulatory compliance document.
Download SDS →✅ Certificate of Analysis (COA)
Lot-specific quality data. Enter your lot number to retrieve the exact COA.
Look up COA →🔬 Specification Sheet
Full quality attributes and acceptance criteria for this grade.
View spec sheet →Advanced Data
Certificates（CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:
Chemical and Physical Properties
| Solubility | DMF(Dimethylformamide) |
|---|
Solution Calculators
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Customer Reviews
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