EMSA Probe PPAR - 10μM

Cat. No.: E745614

GRADE & PURITY 10μM

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

Overview

Aladdin's EMSA Probe - PPAR is a PPAR consensus oligonucleotide for EMSA studies. This double-stranded oligonucleotide contains a well-recognized PPAR binding site, which is common to all three transcription factors, PPARα, PPARβ, and PPARγ, and can be used as an EMSA probe. The sequence of PPAR consensus oligo is as follows

Precautions：

Avoid heating above 40℃, as double-stranded DNA probe will be denatured at high temperatures into single strands that cannot be used in EMSA.For detailed EMSA procedures, please refer to the manual of EMSA kits from .This product is for R&D only. Not for drug, household, or other uses.For your safety and health, please wear a lab coat and disposable gloves during the operation.

Instructions for Use：

1.Set up the probe labeling reaction with isotopes by adding reagents in sequence as follows:Probe to be labeled (1.75µM)2µlT4 Polynucleotide Kinase 10X Buffer1µlNuclease-Free Water5µl[γ-32P]ATP (3,000Ci/mmol at 10mCi/ml)1µlT4 Polynucleotide Kinase (5–10u/µl)1µlTotal volume10µl2.To label the probe with biotin, please referred to the EMSA Probe Biotin Labeling Kit (, GS008).3.For cold competition reaction setup, the amount of unlabeled probe should be 50-100 times that of labeled probe.

Specifications

Specifications & Purity

10μM

Stability And Storage

Store at -20℃ for up to 1 year.

Storage

Store at -20°C

Shipped In

Ice chest + Ice pads

This product requires cold chain shipping. Ground and other economy services are not available.