Evans Blue - Biological Stain, Moligand™ , CAS No.314-13-6

CAS: 314-13-6 Cat. No.: E104208 Formula: C34H24N6Na4O14S4 Molecular Weight: 960.81 Beilstein Registry Number: 16(2)259 EC Number: 206-242-5
AVAILABLE TO ORDER
GRADE & PURITY Biological Stain ? Biological stain grade — dyes characterized for staining cells and tissues. Use in histology and microscopy where staining consistency matters. Moligand™ ? Moligand™ — Aladdin's line of ligands and bioactive small molecules. Use for receptor, pathway, and binding studies needing defined small-molecule tools.
Synonyms
tetrasodium 4-amino-6-[(E)-2-{4'-[(E)-2-(8-amino-1-hydroxy-5,7-disulfonaphthalen-2-yl)diazen-1-yl]-3,3'-dimethyl-[1,1'-biphenyl]-4-yl}diazen-1-yl]-5-hydroxynaphthalene-1,3-disulfonate | Evans blue, sodium salt | CI Direct Blue 53 | EINECS 206-242-5 | 6,6-
Storage
Room temperature,Desiccated
Shipped In
Normal
★
Size
USA
Germany (EU)*
Price
Qty
1g
E104208-1g
8 In stock
—
$9.90
5g
E104208-5g
In stock ≥10
—
$26.90
25g
E104208-25g
7 In stock
—
$89.90
100g
E104208-100g
2 In stock
—
$276.90
Enter a quantity for the sizes you want to add.
🧪
Why this grade

Biological Stain, Moligand™ Biological Stain,Moligand™ for sensitive chromatographic and analytical workflows requiring minimal baseline interference.

🌡

Storage & shipping

Room temperature,Desiccated Ships Normal Check lot-specific COA for exact specifications.

📋

Quality documents

SDS, COA, datasheet, and spec sheet available for download. Lot-specific COA accessible via lot number lookup.

📚

Literature proof

Cited in 130 peer-reviewed publications across chromatography, organic synthesis, and cross-coupling reactions.

Specifications

Synonyms
tetrasodium 4-amino-6-[(E)-2-{4'-[(E)-2-(8-amino-1-hydroxy-5,7-disulfonaphthalen-2-yl)diazen-1-yl]-3,3'-dimethyl-[1,1'-biphenyl]-4-yl}diazen-1-yl]-5-hydroxynaphthalene-1,3-disulfonate | Evans blue, sodium salt | CI Direct Blue 53 | EINECS 206-242-5 | 6,6-
Specifications & Purity
Biological Stain, Moligand™
Biochemical and Physiological Mechanisms
Evans blue is a non-permeating dye. In presence of plasma membrane damage, the dye enters in the cytoplasm and nucleus, thereby staining them blue. Evans blue is used for checking cell viability. It is also used to study BBB (blood-brain barrier) permeabi
Storage
Room temperature,Desiccated
Shipped In
Normal
Grade
Biological Stain, Moligand™
Note
Wherever possible, you should prepare and use solutions on the same day. However, if you need to make up stock solutions in advance, we recommend that you store the solution as aliquots in tightly sealed vials at -20°C. Generally, these will be useable for up to one month. Before use, and prior to opening the vial we recommend that you allow your product to equilibrate to room temperature for at least 1 hour. Need more advice on solubility, usage and handling? Please visit our frequently asked questions (FAQ) page for more details.
Names and Identifiers
Pubchem Sid
Pubchem Sid Url
https://pubchem.ncbi.nlm.nih.gov/substance/488180975
Canonical Smiles
CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+]
IUPAC Name
tetrasodium;4-amino-6-[[4-[4-[(8-amino-1-hydroxy-5,7-disulfonatonaphthalen-2-yl)diazenyl]-3-methylphenyl]-2-methylphenyl]diazenyl]-5-hydroxynaphthalene-1,3-disulfonate
InChIKey
ATNOAWAQFYGAOY-UHFFFAOYSA-J
INCHI
1S/C34H28N6O14S4.4Na/c1-15-11-17(3-7-21(15)37-39-23-9-5-19-25(55(43,44)45)13-27(57(49,50)51)31(35)29(19)33(23)41)18-4-8-22(16(2)12-18)38-40-24-10-6-20-26(56(46,47)48)14-28(58(52,53)54)32(36)30(20)34(24)42;;;;/h3-14,41-42H,35-36H2,1-2H3,(H,43,44,45)(H,46,47,48)(H,49,50,51)(H,52,53,54);;;;/q;4*+1/p-4
Isomeric SMILES
CC1=C(C=CC(=C1)C2=CC(=C(C=C2)N=NC3=C(C4=C(C=C3)C(=CC(=C4N)S(=O)(=O)[O-])S(=O)(=O)[O-])O)C)N=NC5=C(C6=C(C=C5)C(=CC(=C6N)S(=O)(=O)[O-])S(=O)(=O)[O-])O.[Na+].[Na+].[Na+].[Na+]
WGK Germany
3
RTECS
QJ6440000
Molecular Weight
960.81
Beilstein
16(2)259
Reaxy-Rn
7110511
Reaxys-RN_link_address
https://www.reaxys.com/reaxys/secured/hopinto.do?context=S&query=IDE.XRN=7110511&ln=

Documentation

📋 Safety Data Sheet (SDS)

Comprehensive hazard, handling, storage, and regulatory compliance document.

Download SDS →

✅ Certificate of Analysis (COA)

Lot-specific quality data. Enter your lot number to retrieve the exact COA.

Look up COA →

📊 Datasheet

Quick-reference summary of product specifications and applications.

View datasheet →

🔬 Specification Sheet

Full quality attributes and acceptance criteria for this grade.

View spec sheet →

Advanced Data

Taxonomic Classification

Taxonomy Tree

KingdomOrganic compounds
SuperclassBenzenoids
ClassBenzene and substituted derivatives
SubclassBiphenyls and derivatives
Intermediate Tree Nodes Benzidines
Direct Parent3,3'-disubstituted benzidines
Alternative Parents 1-naphthalene sulfonates  1-naphthalene sulfonic acids and derivatives  2-naphthalene sulfonates  2-naphthalene sulfonic acids and derivatives  1-sulfo,2-unsubstituted aromatic compounds  Toluenes  Phenoxides  Sulfonyls  Organosulfonic acids  Azo compounds  Propargyl-type 1,3-dipolar organic compounds  Organooxygen compounds  Organic sodium salts  Organic oxides  Hydrocarbon derivatives  Primary amines  Organopnictogen compounds  
Molecular FrameworkAromatic homopolycyclic compounds
Substituents 3,3'-disubstituted benzidine - Naphthalene sulfonate - 1-naphthalene sulfonic acid or derivatives - 2-naphthalene sulfonic acid or derivatives - Naphthalene sulfonic acid or derivatives - 1-naphthalene sulfonate - 2-naphthalene sulfonate - Naphthalene - Arylsulfonic acid or derivatives - 1-sulfo,2-unsubstituted aromatic compound - Phenoxide - Toluene - Organic sulfonic acid or derivatives - Organosulfonic acid or derivatives - Organosulfonic acid - Sulfonyl - Azo compound - Propargyl-type 1,3-dipolar organic compound - Organic 1,3-dipolar compound - Organic alkali metal salt - Organic salt - Organopnictogen compound - Organic oxide - Primary amine - Amine - Organosulfur compound - Organooxygen compound - Organonitrogen compound - Organic oxygen compound - Hydrocarbon derivative - Organic nitrogen compound - Organic sodium salt - Aromatic homopolycyclic compound
DescriptionThis compound belongs to the class of organic compounds known as 3,3'-disubstituted benzidines. These are organic compounds containing a benzidine skeleton, which is substituted only at the 3- and 3'-positions.
External Descriptors organic sodium salt
3D Structure
Interactive Chemical Structure Model





Associated Targets(Human)
ALB Tchem Serum albumin (2651 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
NFKB1 Tclin Nuclear factor NF-kappa-B p105 subunit (1459 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ATH-8 cell line (300 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ENPP2 Tchem Autotaxin (2645 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ENPP6 Tbio Ectonucleotide pyrophosphatase/phosphodiesterase family member 6 (69 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
ENPP7 Tbio Ectonucleotide pyrophosphatase/phosphodiesterase family member 7 (69 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TDP1 Tchem Tyrosyl-DNA phosphodiesterase 1 (345557 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
HSPD1 Tbio HSP60/HSP10 (366 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
TST Tchem Thiosulfate sulfurtransferase (144 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Associated Targets(non-human)
Ptpn1 Protein-tyrosine phosphatase 1B (270 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Slc17a8 Vesicular glutamate transporter 3 (89 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
PTP1 Protein-tyrosine phosphatase 1 (26 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Luciferin 4-monooxygenase (66902 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
rep Replicase polyprotein 1ab (378 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
groL GroEL/GroES (1042 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
groEL 60 kDa chaperonin (64 Activities)
Activity TypeRelationActivity valueUnitsAction TypeJournalPubMed IddoiAssay Aladdin ID
Mechanisms of Action
Certificates(CoA,COO,BSE/TSE and Analysis Chart)
C of A & Other Certificates(BSE/TSE, COO):
Analytical Chart:

Find and download the COA for your product by matching the lot number on the packaging.

33 results found

Lot NumberCertificate TypeDateItem
L2206584Certificate of AnalysisSep 01, 2026 E104208
L2206763Certificate of AnalysisSep 01, 2026 E104208
L2206774Certificate of AnalysisSep 01, 2026 E104208
L2206772Certificate of AnalysisSep 01, 2026 E104208
D2616395Certificate of AnalysisApr 02, 2026 E104208
D2616396Certificate of AnalysisApr 02, 2026 E104208
D2616394Certificate of AnalysisApr 02, 2026 E104208
D2616393Certificate of AnalysisApr 02, 2026 E104208
C2513421Certificate of AnalysisFeb 27, 2025 E104208
C2513420Certificate of AnalysisFeb 27, 2025 E104208
C2513419Certificate of AnalysisFeb 27, 2025 E104208
C2513418Certificate of AnalysisFeb 27, 2025 E104208
J2422419Certificate of AnalysisOct 10, 2024 E104208
J2422480Certificate of AnalysisOct 10, 2024 E104208
J2422482Certificate of AnalysisOct 10, 2024 E104208
C2418046Certificate of AnalysisOct 22, 2022 E104208
L2206770Certificate of AnalysisOct 22, 2022 E104208
L2315044Certificate of AnalysisOct 22, 2022 E104208
L2206710Certificate of AnalysisOct 22, 2022 E104208
L2206705Certificate of AnalysisOct 22, 2022 E104208
H2430015Certificate of AnalysisOct 22, 2022 E104208
L2206688Certificate of AnalysisOct 22, 2022 E104208
F2214150Certificate of AnalysisMay 25, 2022 E104208
L2315045Certificate of AnalysisMay 25, 2022 E104208
F2214151Certificate of AnalysisMay 25, 2022 E104208
B2317212Certificate of AnalysisMay 25, 2022 E104208
F2214149Certificate of AnalysisMay 25, 2022 E104208
F2214148Certificate of AnalysisMay 25, 2022 E104208
D2419069Certificate of AnalysisMay 25, 2022 E104208
C2418048Certificate of AnalysisMay 25, 2022 E104208
B2317236Certificate of AnalysisMay 25, 2022 E104208
B2317213Certificate of AnalysisMay 25, 2022 E104208
I2227117Certificate of AnalysisOct 30, 2021 E104208
Chemical and Physical Properties
SolubilitySoluble in water.
Molecular Weight960.800 g/mol
XLogP3
Hydrogen Bond Donor Count4
Hydrogen Bond Acceptor Count20
Rotatable Bond Count5
Exact Mass959.982 Da
Monoisotopic Mass959.982 Da
Topological Polar Surface Area404.000 Ų
Heavy Atom Count62
Formal Charge0
Complexity1790.000
Isotope Atom Count0
Defined Atom Stereocenter Count0
Undefined Atom Stereocenter Count0
Defined Bond Stereocenter Count0
Undefined Bond Stereocenter Count0
The total count of all stereochemical bonds0
Covalently-Bonded Unit Count5
Documents & Articles
Biological Stain
Principles and methods of smear staining, microbiological staining, and fundamental dye systems
Quick Reference Guide to “Blue” Reagents in Biological Experiments: Use Cases, Critical Control Points, and Selection Guidance
Adrenomedullin (ADM): Molecular Features, Signaling Mechanisms, and Research and Translational Applications of a Multisystem Vasoactive Peptide
Neuropathological Mechanisms of Cerebral Infarction
Evaluation of Blood-Brain Barrier Permeability: Tracer Extravasation, TEER Measurement, and Interpretation of In Vitro Model Results
Citations of This Product
Solution Calculators
Reviews

Customer Reviews

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