After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
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After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
·Reviewed·Published ·Updated ·8 min read
After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?
The blistering of cell membrane may be caused by too long fixation time, you can shorten the fixation time appropriately to optimize the fixation result.
Da — when not otherwise indicated, molecular weight units are daltons. Mw — weight-average molecular weight. Mn — number-average molecular weight.
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Cite this article
Aladdin Scientific. "After fixing the cells at room temperature for 15 min with this 4% paraformaldehyde, the cell membrane blisters, do I need to change the fixation temperature to 4°C?" Aladdin Knowledge Base, updated Aug 28, 2024. https://www.aladdinsci.com/us_en/faqs/after-fixing-the-cells-at-room-temperature-for-15-min-en.html
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