Recombinant Human C8orf89 Protein: Research Guide & Protocol

Recombinant Human C8orf89 Protein is a research-grade recombinant protein derived from the human C8orf89 gene (chromosome 8 open reading frame 89), produced for use in biochemical, functional, and exploratory studies requiring a well-characterized human protein reagent.

Product Name

Recombinant Human C8orf89 Protein

SKU

rp376318

Gene

C8orf89 (Chromosome 8 Open Reading Frame 89)

Protein Origin

Human (Homo sapiens)

Application

Biochemical assays, functional studies, exploratory research

Storage Recommendation

Store at -20°C or -80°C; avoid repeated freeze-thaw cycles

Reference Number

absea#PP-9916

Protein Background and Gene Context

C8orf89 (Chromosome 8 Open Reading Frame 89) is a human protein-coding gene located on chromosome 8. As with many open reading frame (orf) designations, C8orf89 represents a gene whose protein product has been identified at the sequence level but whose precise molecular function and biological role may still be under active investigation by the research community.

Recombinant Human C8orf89 Protein (rp376318) is produced using recombinant expression systems to provide researchers with a consistent, high-purity source of this protein for use in downstream experiments. Understanding the biology of understudied proteins such as C8orf89 is an important frontier in proteomics, functional genomics, and disease biology research.

Note for researchers: Because C8orf89 is classified as a protein of currently limited annotation, researchers are encouraged to consult the latest entries in UniProt, NCBI Gene, and GeneCards databases for the most up-to-date functional annotations prior to experimental design.

Product Specifications and Quality Control

Aladdin Scientific supplies Recombinant Human C8orf89 Protein (rp376318) as a research-grade reagent. All recombinant proteins in the Aladdin catalog are subject to quality control procedures designed to ensure lot-to-lot consistency and suitability for research applications.

Typical quality control assessments for recombinant proteins include:

• Purity analysis: SDS-PAGE and/or HPLC to confirm protein purity, generally targeted at ≥85% or higher depending on the specific product grade.
• Identity confirmation: Western blot or mass spectrometry may be used to confirm protein identity.
• Endotoxin testing: LAL (Limulus Amebocyte Lysate) assay to ensure endotoxin levels are within acceptable limits for cell-based assays.
• Activity verification: Where applicable, functional activity assays may be performed.

Review flag: Researchers should consult the specific product datasheet for rp376318 for confirmed purity values, formulation details, molecular weight, and expression system information, as these specifications are product-lot specific and not fully detailed in the current source material.

Reconstitution, Handling, and Storage Protocol

Proper handling and storage of recombinant proteins is essential to maintain biological activity and ensure reproducible experimental results. Follow the guidelines below when working with Recombinant Human C8orf89 Protein (rp376318):

Reconstitution

• Centrifuge the vial briefly (1,000–2,000 × g, 30 seconds) before opening to collect any lyophilized material at the bottom of the tube.
• Reconstitute lyophilized protein in the buffer recommended on the product datasheet (typically sterile PBS or water for injection). Add the reconstitution buffer slowly along the tube wall to avoid foaming.
• Gently mix by pipetting or swirling. Do NOT vortex vigorously, as this can denature the protein.
• Allow the protein to dissolve completely at room temperature for 10–15 minutes before use.

Storage

• For short-term use (up to 1 week): store at 4°C.
• For long-term storage: aliquot into single-use volumes and store at -20°C or -80°C.
• Avoid repeated freeze-thaw cycles, as these can reduce protein integrity and activity.
• Addition of a carrier protein (e.g., 0.1% BSA) may improve stability during long-term storage.

General Handling Precautions

• Work on ice when handling reconstituted protein.
• Use low-binding microtubes to minimize protein adsorption losses.
• Verify protein concentration by BCA or Bradford assay upon reconstitution.

Note: Always refer to the product-specific datasheet for rp376318 for formulation-specific reconstitution volumes and storage buffer composition.

Recommended Applications and Experimental Tips

Recombinant Human C8orf89 Protein (rp376318) is intended for research use only and is not approved for diagnostic or therapeutic applications. Potential research applications include:

• Protein–protein interaction studies: Use as bait or prey in pull-down assays, co-immunoprecipitation (Co-IP), or yeast two-hybrid screens to identify binding partners.
• Antibody validation: Serve as a positive control antigen for validating anti-C8orf89 antibodies in Western blot, ELISA, or immunofluorescence assays.
• Biochemical assays: Substrate or cofactor in enzymatic assays if functional data emerge from the literature.
• Structural studies: Input material for X-ray crystallography, cryo-EM, or NMR studies aimed at resolving the three-dimensional structure of C8orf89.
• Cell-based assays: Exogenous addition to cell culture systems to assess functional impact, provided endotoxin levels are verified acceptable.

Experimental tips:

• Always include a negative (buffer-only) and, where available, a positive control in functional assays.
• Titrate the protein concentration carefully, starting from the manufacturer's recommended range and optimizing for your specific assay system.
• Consult primary literature and databases such as STRING, BioGRID, and the Human Protein Atlas for known or predicted interaction networks to inform experimental design.

Ordering and Technical Support

Recombinant Human C8orf89 Protein is available from Aladdin Scientific under SKU rp376318. To order or request a quotation, please contact your regional Aladdin Scientific representative or visit the Aladdin Scientific website.

Technical support: For application-specific advice, protocol optimization, or custom protein production inquiries, Aladdin Scientific's technical support team is available to assist. When contacting support, please have the following information ready:

• Product SKU (rp376318)
• Lot number (found on the product vial label)
• Intended application and experimental system
• Observed issue or question in detail

Custom services: Aladdin Scientific also offers custom recombinant protein production services. If you require C8orf89 variants (e.g., truncations, point mutants, or alternative tags), please enquire with the technical team regarding feasibility and lead times.

Returns and quality complaints: If you receive a product that does not meet the stated specifications, please contact Aladdin Scientific customer service promptly, retaining the original packaging and maintaining cold-chain storage until resolution instructions are provided.

Frequently asked questions

What expression system is used to produce Recombinant Human C8orf89 Protein (rp376318)?

The specific expression system used for rp376318 (e.g., E. coli, baculovirus/insect cells, HEK293, or CHO cells) is detailed on the product datasheet. Please refer to the datasheet or contact Aladdin Scientific technical support for confirmed expression system information, as this directly impacts post-translational modifications and downstream application suitability.

How should I reconstitute and store Recombinant Human C8orf89 Protein to maintain its stability?

Reconstitute the lyophilized protein in the buffer specified on the product datasheet by adding the buffer slowly along the vial wall and mixing gently — do not vortex. For short-term use, store at 4°C for up to one week. For long-term storage, aliquot into single-use volumes and keep at -20°C or -80°C to avoid repeated freeze-thaw cycles, which can compromise protein integrity.

Is Recombinant Human C8orf89 Protein suitable for use in cell-based assays?

Recombinant Human C8orf89 Protein (rp376318) may be used in cell-based assays provided that endotoxin levels are verified to be within acceptable limits prior to use, as endotoxin contamination can confound cell-based results. Please verify the endotoxin specification on the product datasheet and perform a LAL test if necessary before adding the protein to cell culture systems.

What are the known or predicted biological functions of C8orf89?

C8orf89 is designated as a chromosome 8 open reading frame protein, and its precise biological function is not yet fully established in the literature. Researchers are encouraged to consult up-to-date resources such as UniProt, NCBI Gene, and GeneCards for the latest functional annotations, as well as interaction databases such as STRING for predicted protein interaction networks.

Can I use rp376318 as a positive control for anti-C8orf89 antibody validation?

Yes, Recombinant Human C8orf89 Protein (rp376318) is well suited as a positive control antigen for validating anti-C8orf89 antibodies in assays such as Western blot and ELISA. Ensure that the epitope recognized by the antibody of interest corresponds to a region present in the recombinant protein construct by checking the construct boundaries on the product datasheet.

References

1. UniProt Consortium. UniProt: the Universal Protein Knowledgebase. Nucleic Acids Research. Search entry for C8orf89 (Human).
2. NCBI Gene Database. Gene entry for C8orf89, Homo sapiens. National Center for Biotechnology Information.
3. GeneCards. C8orf89 Gene Card. Weizmann Institute of Science.
4. Thermo Fisher Scientific. Protein Reconstitution and Storage Guidelines. Technical Resource Library.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.