Recombinant Human GMPR Protein: Function & Research Use

Recombinant Human GMPR Protein is a recombinantly produced form of the human guanosine 5′-monophosphate reductase enzyme, essential for catalyzing the NADPH-dependent reductive deamination of GMP to IMP within the purine nucleotide interconversion pathway.

Protein Name

Recombinant Human GMPR Protein

Gene Name

GMPR

Enzymatic Function

GMP reductase; catalyzes NADPH-dependent reductive deamination of GMP to IMP

Pathway

Purine nucleotide interconversion (GMP → IMP)

Host System

Recombinant expression system (refer to product datasheet for details)

Research Applications

Enzyme activity assays, nucleotide metabolism studies, protein interaction studies

SKU

rp365867

What Is GMPR and Why Does It Matter?

Guanosine 5′-monophosphate reductase (GMPR) is an enzyme encoded by the GMPR gene in humans. It catalyzes the irreversible, NADPH-dependent reductive deamination of guanosine monophosphate (GMP) to inosine monophosphate (IMP), a pivotal step that links the GMP pool to the broader purine nucleotide interconversion network. By governing the balance between guanine and adenine nucleotides, GMPR plays a fundamental role in maintaining intracellular nucleotide homeostasis.

Dysregulation of purine nucleotide metabolism has been associated with a range of pathological states, including proliferative diseases and immune dysfunction, making GMPR a protein of considerable interest in both basic and translational research contexts. Understanding GMPR enzymatic kinetics and regulatory mechanisms is therefore important for researchers investigating nucleotide biosynthesis, cell proliferation, and potential therapeutic targets.

Biochemical Properties of Recombinant Human GMPR Protein

Recombinant Human GMPR Protein is produced using recombinant expression technology to deliver a consistent, research-grade enzyme suitable for in vitro experimentation. The native GMPR protein functions as a homotetramer, with each subunit containing a TIM-barrel fold characteristic of the amidohydrolase superfamily. The active site coordinates NADPH binding and substrate recognition to facilitate catalysis.

Key biochemical parameters — including molecular weight, purity, formulation buffer, and storage conditions — should be confirmed against the current product datasheet (SKU: rp365867), as these specifications may be updated. Researchers are advised to centrifuge the vial briefly before use, aliquot to avoid repeated freeze-thaw cycles, and store according to supplier-recommended conditions to preserve enzymatic activity.

Research Applications

Recombinant Human GMPR Protein is suited to a variety of experimental applications in biochemistry and cell biology:

• Enzyme Activity Assays: The recombinant protein can be used as an active enzyme source to measure GMP reductase activity spectrophotometrically by monitoring NADPH oxidation at 340 nm.
• Nucleotide Metabolism Studies: GMPR serves as a tool for dissecting purine nucleotide interconversion pathways, enabling researchers to probe how cells regulate the GMP-to-IMP conversion under various physiological or pharmacological conditions.
• Protein–Protein Interaction Studies: The recombinant protein can be employed in pull-down assays or surface plasmon resonance experiments to identify binding partners and regulatory modulators of GMPR.
• Drug Discovery and Target Validation: As a metabolic enzyme with relevance to cell proliferation, GMPR is of interest for screening inhibitor libraries and validating it as a therapeutic target in relevant disease models.
• Structural Biology: Purified recombinant GMPR can support crystallography or cryo-EM studies aimed at resolving substrate-binding conformations and understanding allosteric regulation.

Handling, Storage, and Quality Considerations

To ensure reproducible results, proper handling of Recombinant Human GMPR Protein is essential. Researchers should observe the following best practices:

• Avoid repeated freeze-thaw cycles: Aliquot the protein upon first thaw to preserve activity and prevent aggregation.
• Storage temperature: Refer to the product datasheet (SKU: rp365867) for the recommended storage temperature, typically −70 °C or −20 °C depending on formulation.
• Carrier protein: If the protein is supplied without a stabilizing carrier, consider adding BSA for long-term storage aliquots.
• Quality verification: Confirm purity by SDS-PAGE and biological activity by an enzyme activity assay prior to use in critical experiments.
• Lot-to-lot consistency: When comparing data across experiments, note the lot number and confirm specifications with the accompanying Certificate of Analysis (CoA).

For specific formulation details, purity grade, endotoxin levels, and confirmed activity data, consult the current product datasheet available from Aladdin Scientific.

Frequently asked questions

What is the enzymatic function of GMPR?

GMPR (guanosine 5′-monophosphate reductase) catalyzes the NADPH-dependent reductive deamination of GMP to IMP. This reaction is a key step in the purine nucleotide interconversion pathway, helping to regulate the intracellular balance between guanine and adenine nucleotide pools.

What are the recommended storage conditions for Recombinant Human GMPR Protein?

Storage conditions depend on the specific formulation supplied. Researchers should consult the product datasheet for SKU rp365867 for confirmed temperature, buffer, and stabilizer recommendations. As a general practice, avoid repeated freeze-thaw cycles by aliquoting the protein upon first thaw.

In which experimental assays can Recombinant Human GMPR Protein be used?

Recombinant Human GMPR Protein is suitable for enzyme activity assays (monitoring NADPH oxidation at 340 nm), nucleotide metabolism pathway studies, protein–protein interaction analyses (pull-down, SPR), inhibitor screening for drug discovery, and structural biology applications such as X-ray crystallography or cryo-EM.

How should I verify the quality of Recombinant Human GMPR Protein before use?

Purity should be confirmed by SDS-PAGE, and biological activity should be verified using a GMP reductase enzyme activity assay. Refer to the Certificate of Analysis (CoA) provided with each lot for purity percentage, endotoxin levels, and activity data specific to your batch.

Is Recombinant Human GMPR Protein suitable for use in drug discovery programs?

Yes. Because GMPR regulates nucleotide pools that influence cell proliferation, it is considered a relevant metabolic target. The recombinant protein can be used in high-throughput enzymatic inhibitor screens and for target validation studies in disease-relevant cell-based models.

References

1. Hedstrom L. IMP dehydrogenase: structure, mechanism, and inhibition. Chemical Reviews. 2009;109(7):2903-2928. doi:10.1021/cr900021w
2. Sintchak MD, Nimmesgern E. The structure of inosine 5′-monophosphate dehydrogenase and the design of novel inhibitors. Immunopharmacology. 2000;47(2-3):163-184. doi:10.1016/S0162-3109(00)00193-4
3. Aladdin Scientific. Recombinant Human GMPR Protein, SKU rp365867. Product Datasheet.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.