Recombinant Human MCOLN1 Protein: FAQs & Key Facts

Recombinant Human MCOLN1 Protein is a research-grade reagent representing the human mucolipin-1 channel protein, widely used to study lysosomal function, intracellular calcium signaling, and the molecular basis of mucolipidosis type IV.

Product Name

Recombinant Human MCOLN1 Protein

SKU

rp368115

Gene / Protein

MCOLN1 (Mucolipin-1, TRPML1)

Protein Family

Transient receptor potential mucolipin (TRPML) channel family

Primary Localization

Lysosomal membrane

Research Applications

Lysosomal biology, calcium signaling, mucolipidosis type IV disease modeling, autophagy research

Supplier

Aladdin Scientific

What Is MCOLN1 and Why Is It Important in Research?

MCOLN1 (Mucolipin-1), also known as TRPML1, is a member of the transient receptor potential mucolipin (TRPML) subfamily of ion channels. It is primarily localized to the lysosomal membrane, where it functions as a calcium-permeable cation channel regulating intracellular calcium release from lysosomes. MCOLN1 plays critical roles in lysosomal biogenesis, membrane trafficking, autophagy, and exocytosis.

Loss-of-function mutations in the MCOLN1 gene are the cause of mucolipidosis type IV (MLIV), a rare autosomal recessive lysosomal storage disorder characterized by psychomotor retardation and ophthalmological abnormalities. Recombinant Human MCOLN1 Protein enables researchers to study the channel's structure-function relationships, its interacting partners, and potential therapeutic targets for MLIV and related lysosomal diseases.

What Research Applications Is Recombinant Human MCOLN1 Protein Suitable For?

Recombinant Human MCOLN1 Protein is a versatile tool for a range of biochemical and cell-biology applications, including but not limited to:

• Protein-protein interaction studies: Identifying novel binding partners of MCOLN1 using pull-down assays or co-immunoprecipitation.
• Structural and biophysical studies: Supporting crystallography, cryo-EM, or in vitro binding assays to characterize channel conformation and gating.
• Antibody validation: Serving as a positive control antigen to validate MCOLN1-specific antibodies in Western blotting or ELISA.
• Disease mechanism research: Modeling the molecular consequences of MCOLN1 dysfunction relevant to mucolipidosis type IV and other lysosomal storage disorders.
• Drug discovery: Screening small-molecule modulators or evaluating potential therapeutic compounds targeting the TRPML1 channel.

Researchers should confirm suitability for specific assay formats by consulting the product datasheet or contacting Aladdin Scientific technical support.

Handling, Storage, and Stability Recommendations

Proper handling and storage of recombinant proteins are essential to maintain activity and integrity. The following general guidelines apply to Recombinant Human MCOLN1 Protein; always refer to the specific product datasheet (SKU: rp368115) for lot-specific conditions:

• Storage temperature: Store at −80°C for long-term storage. Avoid repeated freeze-thaw cycles, which can cause protein aggregation and loss of activity.
• Aliquoting: Upon first thaw, aliquot the protein into single-use volumes to minimize freeze-thaw cycles.
• Working conditions: Keep on ice during experimental setup. Use within the timeframe recommended on the datasheet after initial thawing.
• Buffer compatibility: Consult the datasheet for formulation details. Avoid introducing detergents or denaturants incompatible with downstream assays.
• Contamination prevention: Use sterile, low-binding tubes and pipette tips to prevent adsorption or microbial contamination.

If you observe precipitation or unexpected activity loss, contact Aladdin Scientific technical support for guidance.

Quality, Purity, and Regulatory Considerations

Recombinant Human MCOLN1 Protein supplied by Aladdin Scientific (SKU: rp368115) is manufactured as a research-use-only (RUO) reagent. Key quality considerations include:

• Purity: Specific purity levels and analytical data (e.g., SDS-PAGE, HPLC) are provided on the product datasheet. Researchers should review the certificate of analysis (CoA) supplied with each lot.
• Endotoxin levels: Endotoxin content is typically measured and reported; consult the datasheet or CoA for lot-specific values, particularly important for cell-based assays.
• Not for therapeutic use: This product is strictly intended for in vitro research and is not approved for use in humans, clinical diagnostics, or veterinary applications.
• Lot-to-lot consistency: Contact Aladdin Scientific for lot reservation or to request data from specific lots if consistency across experiments is critical.

Ordering and Technical Support for MCOLN1 Protein

Recombinant Human MCOLN1 Protein (SKU: rp368115) is available for order directly through the Aladdin Scientific website or by contacting our sales team. When placing an order, please have the following information ready:

• Product SKU: rp368115
• Desired quantity and pack size
• Shipping address and any special cold-chain requirements
• Purchase order number or institutional billing details

For technical inquiries—including questions about assay suitability, protein formulation, or troubleshooting—please contact Aladdin Scientific technical support. Our team can provide guidance on experimental design and help ensure you receive the most appropriate product for your research needs.

Volume discounts and custom formulation requests may be available; please inquire with our sales representatives for details.

Frequently asked questions

What is the biological function of MCOLN1 (Mucolipin-1)?

MCOLN1, also called TRPML1, is a calcium-permeable cation channel located predominantly on the lysosomal membrane. It regulates calcium release from lysosomes, which is critical for lysosomal biogenesis, membrane trafficking, autophagy flux, and lysosomal exocytosis. Loss-of-function mutations in MCOLN1 cause mucolipidosis type IV, a severe lysosomal storage disorder.

What expression system is used to produce Recombinant Human MCOLN1 Protein (rp368115)?

The specific expression system used for Recombinant Human MCOLN1 Protein (SKU: rp368115) is detailed in the product datasheet. Common systems for recombinant membrane-associated proteins include mammalian, insect (baculovirus), or E. coli expression platforms. Please refer to the datasheet or contact Aladdin Scientific for confirmed expression system details for this lot.

How should Recombinant Human MCOLN1 Protein be stored to maintain its activity?

For long-term storage, keep Recombinant Human MCOLN1 Protein at −80°C. Upon first thaw, aliquot the protein into single-use volumes and avoid repeated freeze-thaw cycles, which can lead to aggregation and loss of biological activity. Always refer to the specific lot's datasheet for precise storage and stability recommendations.

Is Recombinant Human MCOLN1 Protein (rp368115) suitable for use in cell-based assays?

Recombinant Human MCOLN1 Protein may be used in certain cell-based assay formats, but suitability depends on the specific experimental design and endotoxin levels of the lot. Researchers conducting cell-based work should check the certificate of analysis for endotoxin content and consult Aladdin Scientific technical support to confirm compatibility with their assay system.

Can Recombinant Human MCOLN1 Protein be used as an antigen for antibody validation?

Yes, recombinant proteins such as MCOLN1 are commonly used as positive control antigens to validate the specificity of MCOLN1-targeting antibodies in assays such as Western blot and ELISA. Confirm that the recombinant protein sequence matches the epitope region recognized by your antibody of interest before use.

References

1. Bargal R, et al. Identification of the gene causing mucolipidosis type IV. Nature Genetics. 2000.
2. Cheng X, et al. The intracellular Ca2+ channel MCOLN1 is required for sarcolemma repair to prevent muscular dystrophy. Nature Medicine. 2014.
3. Xu H, Ren D. Lysosomal physiology. Annual Review of Physiology. 2015.

Products are supplied for research and development use only. Not for use in humans, animals, diagnosis, or therapy.