Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Maleimide-PEG modified drug can be conjugated to the antibody at the pH 7.5 buffer solution contained 50 mM sodium phosphate, 50 mM sodium chloride and 2 mM EDTA The disulfide bridges in the antibody were then reduced with various molar equivalents of DTT at 30 ̊C for 30 min to afford different ...
Updated Apr 202512 min read✓ Reviewed
Related topics
Techniques often explored alongside immunological experiments.
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