Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
This article primarily refers to the study by Badland et al., entitled “A comparative study of amide-bond forming reagents in aqueous media—Substrate scope and reagent compatibility,” published in Tetrahedron Letters.
The most familiar route from nitro compounds to amides is usually to first reduce the nitro group to an amine, and then react that amine with a carboxylic acid derivative or an activated carboxylic acid system. This route is mature, reliable, and remains the most common choice in both laboratory ...
Amide bond formation is one of the most common fundamental transformations in the synthesis of drug molecules, natural products, and functional molecules. In efforts to improve amidation reactions, researchers are concerned not only with whether a bond can be formed, but also with whether the ...
Amide bond formation is one of the most common fundamental transformations in organic synthesis, medicinal chemistry, and the preparation of functional molecules. In efforts to improve amidation reactions, the central questions have remained the same: can additional activation steps be reduced, ...
Techniques often explored alongside immunological experiments.
