Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Isotype control antibody is a negative control reagent that has the same immunoglobulin class, subclass, and light chain type as the research antibody (experimental antibody) but does not bind to the target antigen.
Flow cytometry is a powerful technique that is often used to analyze surface and internal antigens of cells. When detecting intracellular antigens, cells usually need to be fixed and permeabilized.
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