Gel electrophoresis

In agarose and polyacrylamide gel electrophoresis, DNA and RNA do not inherently exhibit visible color or readily detectable fluorescence signals; therefore, nucleic acid stains are required for band visualization. Major classes of nucleic acid dyes differ substantially in binding mechanism, ...

Standard Operating Procedure (SOP) for Bis-Tris Polyacrylamide Gel Electrophoresis (SDS-PAGE)

Gel electrophoresis is a common laboratory technique in molecular biology to identify, quantify, and purify nucleic acids. Because of its speed, simplicity, and versatility, the method is widely employed for separation and analysis of nucleic acids. Using gel electrophoresis, nucleic acids in ...

The DNA of apoptotic cells is cut into fragments of about 200 base pairs under the action of caspase-activated nuclease (CAD), forming DNA ladders. By detecting DNA fragmentation during apoptosis, the degree of apoptosis can be evaluated.