Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
Choline metabolism connects cell membrane phospholipid synthesis, methyl donor cycling, cholinergic neurotransmitter generation, hepatic lipid transport, and gut microbiota-related metabolism. Choline metabolism detection should not be limited to free choline alone. Depending on the research ...
The core of carbon-nitrogen metabolic crosstalk lies in the dynamic conversion between carbon skeletons and nitrogen flow. Glutamate and α-ketoglutarate form key hubs connecting the TCA cycle, transamination reactions, glutamine metabolism, and amino acid biosynthesis. Their changes reflect not ...
β-Hydroxybutyrate is one of the predominant ketone body forms in blood and tissues. It is commonly used to evaluate enhanced fatty acid oxidation, hepatic ketogenesis, starvation or fasting status, diabetic ketosis, exercise metabolism, and mitochondrial energy conversion. Compared with ...
TCA cycle metabolite detection is used to evaluate mitochondrial oxidative metabolism, substrate entry, anaplerotic reactions, redox status, and metabolic reprogramming. Common targets include citrate, α-ketoglutarate, succinate, fumarate, malate, and oxaloacetate. Method selection should be ...
Creatinine content detection is commonly used for renal function assessment, urinary indicator normalization, and drug nephrotoxicity studies in serum, plasma, urine, and animal experimental samples. The core differences among detection methods lie in reaction principle, signal generation mode, ...
Hydroxyproline determination is a classical quantitative technique in collagen-related research and is widely used in fibrosis assessment, tissue-remodeling studies, collagen-material analysis, and pharmacodynamic evaluation.
Techniques often explored alongside immunological experiments.
