Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
The uses of natural biological components derived from Escherichia coli in life science research are not limited to endotoxin stimulation. From native enzymatic readouts and host-background control in immunoassays, to pattern-recognition receptor activation in inflammatory models, and further to ...
Endotoxin commonly refers to lipopolysaccharide (LPS) enriched in the outer leaflet of the outer membrane of Gram-negative bacteria.
Lipopolysaccharide (LPS), a major component of the outer membrane of Gram-negative bacteria, binds to the TLR4 receptor and its co-receptors (e.g., CD14, MD-2) on macrophages, activating NF-κB, MAPK, and other pathways and inducing production/secretion of inflammatory cytokines such as TNF-α, ...
To efficiently remove endotoxins (lipopolysaccharides, LPS) from aqueous protein samples using the temperature-dependent phase separation property of Triton X-114, while retaining the target protein in the aqueous phase.
Lipopolysaccharides (LPS) are essential components of the outer membrane of Gram-negative bacteria, influencing bacterial survival and pathogenicity. LPS is located in the outer layer of the bacterial membrane and is directly exposed on the cell surface in non-capsulated strains. The complex ...
Techniques often explored alongside immunological experiments.
