Techniques for detecting, quantifying and localizing antigens and antibodies — ELISA, Western blotting, immunohistochemistry and flow cytometry. Below are the protocols, FAQs and technical articles in our knowledge base tagged with this topic.
In handling cell and tissue samples, choosing a lysis buffer is not just about “breaking cells open”—it determines whether proteins are detectable, remain active, and retain complexes and post-translational modifications. Different systems vary greatly in strength, denaturation, and ...
After lysing the cells first with the lysate, add 5x upsampling buffer in a 4:1 volume, then water bath denaturation is it better to use a 100 degree water bath or a 95 degree water bath?
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